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Anti-human Interleukin(IL)-4 Clone 8D4-8 Cross-Reacts With Myosin-9 Associated With Apoptotic Cells and Should Not Be Used for Flow Cytometry Applications Querying IL-4 Expression.
Interleukin(IL)-Four is produced by T cells and different leukocytes and is a crucial mediator of monocyte and B cell responses. During routine circulation cytometry panel validation for the investigation of intracellular cytokines, we noticed distinctive IL-4 expression patterns related to the broadly obtainable monoclonal antibody 8D4-8.
Namely, IL-4 (8D4-8) expression was noticed within the absence of mobile activation and enhanced following staurosporine publicity. Mass spectrometry evaluation of immunoprecipitates from peripheral blood lymphocytes (PBL) revealed that 8D4-8 cross-reacts with the ever-present cytoskeletal protein myosin-9.
We confirmed these outcomes by western blotting immunoprecipitates, utilizing immunofluorescence amongst staurosporine-treated Caco-2 cells, and by surface-labeling PBL for 8D4-8 and myosin-9 and analyzing by circulation cytometry. Although beforehand reported from a number of impartial teams, we discovered no proof to help the speculation that IL-4 is produced by apoptotic cells. Rather, this seems to have been myosin-9. Our information point out clone 8D4-8 shouldn’t be used within the circulation cytometric examine of IL-4. Furthermore, our work calls for a reevaluation of earlier circulation cytometric research which have used this clone for IL-4 evaluation and highlights the significance of validation in antibody-based assays.
Anti-human Interleukin(IL)-4 Clone 8D4-8 Cross-Reacts With Myosin-9 Associated With Apoptotic Cells and Should Not Be Used for Flow Cytometry Applications Querying IL-4 Expression.
Anti-EGFR-resistant clones decay exponentially after development: implications for anti-EGFR re-challenge.
<AbstractText>Colorectal most cancers (CRC) has been proven to accumulate RAS and EGFR ectodomain mutations as mechanisms of resistance to epidermal progress issue receptor (EGFR) inhibition (anti-EGFR). After anti-EGFR withdrawal, RAS and EGFR mutant clones lack a progress benefit relative to different clones and decay; nonetheless, the kinetics of decay stay unclear.
We sought to find out the kinetics of acquired RAS/EGFR mutations after discontinuation of anti-EGFR remedy.</AbstractText><AbstractText>We current the post-progression circulating tumor DNA (ctDNA) profiles of 135 sufferers with RAS/BRAF wild-type metastatic CRC handled with anti-EGFR who acquired RAS and/or EGFR mutations throughout remedy. Our validation cohort consisted of an exterior dataset of 73 sufferers with a ctDNA profile suggestive of prior anti-EGFR publicity and serial sampling. A separate retrospective cohort of 80 sufferers was used to guage total response fee and development free survival throughout re-challenge therapies.</AbstractText><p><div><b>RESULTS</b></div>Our evaluation confirmed that RAS and EGFR relative mutant allele frequency decays exponentially (r<sup>2</sup>=0.93 for RAS; r<sup>2</sup>=0.94 for EGFR) with a cumulative half-life of 4.Four months.
We validated our findings utilizing an exterior dataset of 73 sufferers with a ctDNA profile suggestive of prior anti-EGFR publicity and serial sampling, confirming exponential decay with an estimated half-life of 4.three months. A separate retrospective cohort of 80 sufferers confirmed that sufferers had the next total response fee throughout re-challenge therapies after growing time intervals, as predicted by our mannequin.</p><AbstractText>These outcomes present scientific help for anti-EGFR re-challenge and information the optimum timing of re-challenge initiation.</AbstractText>
Primary and secondary anti-viral response captured by the dynamics and phenotype of particular person T cell clones.
The various repertoire of T-cell receptors (TCR) performs a key position within the adaptive immune response to infections. Using TCR alpha and beta repertoire sequencing for T-cell subsets, in addition to single-cell RNAseq and TCRseq, we observe the concentrations and phenotypes of particular person T-cell clones in response to major and secondary yellow fever immunization – the mannequin for acute an infection in people – exhibiting their massive variety. We affirm the secondary response is an order of magnitude weaker, albeit ∼ 10 days quicker than the first one.
Should the Mouse Myeloid Differentiation Factor 88 (MyD88) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Myeloid Differentiation Factor 88 (MyD88) in samples from tissue homogenates, cell lysates or other biological fluids.
Should the Mouse Myeloid Differentiation Factor 88 (MyD88) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Myeloid Differentiation Factor 88 (MyD88) in samples from tissue homogenates, cell lysates or other biological fluids.
Rat Myeloid Differentiation Factor 88 (MyD88) ELISA Kit
Should the Rat Myeloid Differentiation Factor 88 (MyD88) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Myeloid Differentiation Factor 88 (MyD88) in samples from tissue homogenates, cell lysates or other biological fluids.
Rat Myeloid Differentiation Factor 88 (MyD88) ELISA Kit
Should the Rat Myeloid Differentiation Factor 88 (MyD88) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Myeloid Differentiation Factor 88 (MyD88) in samples from tissue homogenates, cell lysates or other biological fluids.
Human Myeloid Differentiation Factor 88 (MyD88) ELISA Kit
Description: A polyclonal antibody against MYD88. Recognizes MYD88 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against MYD88. Recognizes MYD88 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IHC:1/200-1/1000.ELISA:1/20000
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against MYD88. Recognizes MYD88 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200
Description: A polyclonal antibody against MYD88. Recognizes MYD88 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200
Description: A polyclonal antibody against MYD88. Recognizes MYD88 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MYD88 . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MyD88 . This antibody is tested and proven to work in the following applications:
Immunogen information: Synthesized peptide derived from the Internal region of human MyD88
Applications tips:
Description: A polyclonal antibody for detection of MyD88 from Human, Mouse, Rat. This MyD88 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human MyD88
Immunogen information: Synthesized peptide derived from the Internal region of human MyD88
Applications tips:
Description: A polyclonal antibody for detection of MyD88 from Human, Mouse, Rat. This MyD88 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human MyD88
Immunogen information: Synthesized peptide derived from the Internal region of human MyD88
Applications tips:
Description: A polyclonal antibody for detection of MyD88 from Human, Mouse, Rat. This MyD88 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human MyD88
Description: MyD88 is a protein encoded by the MYD88 gene which is approximately 33,2 in kDa. MyD88 is localised to the cytoplasm. It is involved in activated TLR4 signalling and diseases associated with the TLR signalling cascade. It is a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. It functions as an essential signal transducer in the interleukin-1 and toll-like receptor signalling pathways that regulate the activation of numerous proinflammatory genes. MyD88 is expressed ubiquitously expressed in human tissues. Mutations in the MYD88 gene may result in macroglobulinemia and a MyD88 deficiency. STJ94293 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of MyD88 protein.
Description: This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants.
Description: This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants.
Description: A competitive ELISA for quantitative measurement of Rat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Myd88/ Myeloid differentiation primary response protein MyD88 ELISA Kit
Description: A competitive ELISA for quantitative measurement of Porcine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human MYD88/ Myeloid differentiation primary response protein MyD88 ELISA Kit
Description: A competitive ELISA for quantitative measurement of Monkey Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Myd88(Myeloid differentiation primary response protein MyD88) ELISA Kit
Comparisons between Mnoclonal, Polyclonal and Recombinant antibodies and their benefits: Regular monoclonal antibodies have higher purity, better specificity and less lot-to-lot variations than polyclonal antibodies. Recombinant antibodies, however,
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against MYD88. Recognizes MYD88 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against MYD88. Recognizes MYD88 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against MYD88. Recognizes MYD88 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A competitive ELISA for quantitative measurement of Guinea pig Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Myeloid differentiation primary response protein MyD88(MYD88) ELISA kit
Description: A competitive ELISA for quantitative measurement of Guinea pig Myeloid differentiation primary response protein MyD88(MYD88) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MYD88 (aa233-248). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MYD88 (aa233-248). This antibody is tested and proven to work in the following applications:
Estimating the fraction of the T-cell response directed in opposition to the one immunodominant epitope, we establish the sequence options of TCRs that outline the excessive precursor frequency of the 2 main TCR motifs particular for this explicit epitope. We additionally present the consistency of clonal growth dynamics between bulk alpha and beta repertoires, utilizing a brand new methodology to reconstruct alpha-beta pairings from clonal trajectories.
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