Abstract

Peanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour arker  it strongly recognises the most cancers particular T antigen (Galbeta1–>3GalNAc-), however not its sialylated model. However, a further specificity in the direction of Galbeta1–>4GlcNAc (LacNAc), which isn’t tumour particular, had been attributed to PNA.

For right interpretation of lectin histochemical outcomes we examined PNA sugar specificity utilizing naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, fairly than mono- or disaccharides as ligands. Dot-blots, switch blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and different lectins of recognized specificity. Modifications of PNA-binding glycoproteins, together with selective removing of O-linked oligosaccharides and therapy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) in addition to uncovered T antigen (Galbeta1–>Three GalNAc-) was glorious as sugar moiety in glycoproteins for his or her recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Results have been confirmed utilizing TAG-specific human serum anti-alpha-galactoside antibody.

 

Arachis hypogaea (Peanut) Lectin (PNA) – HRP

Affinity-purified PNA is conjugated to horseradish peroxidase (HRP) enzyme. HRP is mostly used for blotting, immunoassays and immunohistochemistry strategies. It is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, leading to a coloured or fluorescent product or launch of sunshine as a byproduct of the response. This product is available in a stabilized liquid type.For substrate use of HRP-labelled protein detection we provide our Intensive ChemiLuminescence (ICLTM) package (SKU: 20810000). ICL is a two-component substrate that incorporates a steady luminol resolution with an enhancer and a steady peroxide buffer resolution. It has a robust mild emission and a sensitivity at picogram stage that’s extremely appropriate for the fast detection of HRP-conjugated proteins. Advantages of chemiluminescent substrates over different detection strategies consists of a number of exposures, blots could also be reprobed, detects and quantitates a variety of protein concentrations, and yields best sensitivity of any obtainable methodology.We additionally provide 3, 3, 5, 5-Tetramethyl benzidine (TMB, SKU: 42000012) and TMB OneTM (TMB 1) HRP substrate for HRP labelled detection. TMB is an acceptable substrate for the immunoassay of horseradish peroxidase and is a noncarcinogenic by-product of benzidine with an absorbance at 450 nm. Oxidation of TMB by HRP yields a coloured product. s TMB 1TM is a one part peroxidase substrate (SKU: 21530073).

arachis eylabs

arachis eylabs

Application

General Western Blot Protocol:

  • Glycoprotein pattern dimension: 500ng
  • Lectin Concentration: 0.1ug/ml
  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS web page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for two hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect utilizing chemiluminescent substrate (CPS1-120)

Biochem/Physiol Actions

PNA doesn’t agglutinate regular human erythrocytes, however strongly agglutinates neuraminidase handled erythrocytes. PNA has potent anti-T exercise just like the anti-T antibody in human sera. The lectin can be utilized to differentiate between human lymphocyte subsets.

Unit Definition

One unit will type 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.Zero at 20 °C.

Physical type

Contains sodium citrate

Preparation Note

Prepared from peroxidase (P8375) utilizing a modification of a printed methodology, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
Arachis hypogaea lectin (PNA
05-0116-50 50 mg
EUR 314
Arachis hypogaea Conglutin-7
1-CSB-EP757666ANE
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 22 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Arachis hypogaea Conglutin-7 expressed in E.coli
Arachis hypogaea Arachin Ahy-3
1-CSB-YP737391ANE
  • EUR 679.00
  • EUR 335.00
  • EUR 2172.00
  • EUR 1051.00
  • EUR 1442.00
  • EUR 435.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 21.9 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Arachis hypogaea Arachin Ahy-3,partial expressed in Yeast
Arachis hypogaea Arachin Ahy-3
1-CSB-EP737391ANE
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 35.9 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Arachis hypogaea Arachin Ahy-3 expressed in E.coli
CF488A Arachis hypogaes Lectin PNA (Peanut)
29060 1mg
EUR 195
Description: Minimum order quantity: 1 unit of 1mg
CF568 Arachis hypogaes Lectin PNA (Peanut)
29061 1mg
EUR 195
Description: Minimum order quantity: 1 unit of 1mg
CF594 Arachis hypogaes Lectin PNA (Peanut)
29062 1mg
EUR 195
Description: Minimum order quantity: 1 unit of 1mg
CF640R Arachis hypogaes Lectin PNA (Peanut)
29063 1mg
EUR 195
Description: Minimum order quantity: 1 unit of 1mg
Arachis hypogaea Allergen Ara h 1, clone P17
1-CSB-YP331779ANE(A4)
  • EUR 586.00
  • EUR 299.00
  • EUR 2172.00
  • EUR 900.00
  • EUR 1442.00
  • EUR 382.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 70.2 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in Yeast
Arachis hypogaea Allergen Ara h 1, clone P17
1-CSB-EP331779ANE(A4)
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 71.7 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in E.coli
Arachis hypogaea Allergen Ara h 1, clone P17
1-CSB-EP331779ANE
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 39 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17,partial expressed in E.coli
Arachis hypogaea Allergen Ara h 1, clone P41B
1-CSB-CF337356ANE
  • EUR 965.00
  • EUR 665.00
  • EUR 715.00
  • 1MG
  • 200ug
  • 500ug
  • MW: 72.8 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B expressed in in vitro E.coli expression system
Peanut agglutinin
AG047 1 mg
EUR 523
Peanut agglutinin
AT047 1mg
EUR 945
Peanut oil
GK9829-100ML 100 ml
EUR 54
Peanut oil
GK9829-1L 1 l
EUR 130
Peanut oil
GK9829-250ML 250 ml
EUR 70
Peanut oil
GK9829-500ML 500 ml
EUR 90
Peanut Protein Antibody
10-2833 1 mg
EUR 300
Description: Peanut Protein Monoclonal
Peanut Protein Antibody
10-2834 1 mg
EUR 300
Description: Peanut Protein Monoclonal
Peanut Protein Antibody
10-2835 1 mg
EUR 300
Description: Peanut Protein Monoclonal
Peanut Protein Antibody
10-2836 1 mg
EUR 300
Description: Peanut Protein Monoclonal
Peanut Protein Antibody
10-2837 1 mg
EUR 300
Description: Peanut Protein Monoclonal