Nathan Holland

Preparation of a novel antiserum to aromatase with high affinity and specificity: Its clinicopathological significance on breast cancer tissue.

Aromatase inhibitors have been broadly used for the endocrine therapy of estrogen-dependent breast cancer in postmenopausal sufferers. However, clinicopathological research of aromatase have been restricted due to unsatisfactory specificity and/or restricted availability of anti-aromatase antibodies. Here, we have now generated a polyclonal antiserum with high affinity and specificity for human Read more…

By Nathan Holland, ago

A Novel Antiserum Against a Predicted Human Peripheral Choline Acetyltransferase (hpChAT) for Labeling Neuronal Structures in Human Colon.

Choline acetyltransferase (ChAT), the enzyme synthesizing acetylcholine (ACh), has an exon-skipping splice variant which is expressed preferentially in the peripheral nervous system (PNS) and thus termed peripheral ChAT (pChAT). A rabbit antiserum beforehand produced towards rat pChAT (rpChAT) has been used for immunohistochemistry (IHC) to research peripheral cholinergic buildings in Read more…

By Nathan Holland, ago

Four amino acids as serum biomarkers for anti-asthma effects in the ovalbumin-induced asthma mouse model treated with extract of Asparagus cochinchinensis.

The butanol extract of <i>Asparagus cochinchinensis</i> roots fermented with <i>Weissella cibaria</i> (BAW) successfully prevents irritation and transforming of airway within the ovalbumin (OVA)-induced bronchial asthma mannequin. To characterize biomarkers that may predict the anti-asthmatic results induced by BAW therapy, we measured the alteration of endogenous metabolites within the serum of Read more…

By Nathan Holland, ago

CD19-targeting fusion protein combined with PD1 antibody enhances anti-tumor immunity in mouse models.

In our earlier research, utilizing a B cell vaccine (scFv-Her2), the concentrating on of tumor-associated antigen Her2 (human epidermal progress issue receptor-2) to B cells by way of the anti-CD19 single chain variable fragment (scFv) was proven to enhance tumor-specific immunity, which enhanced tumor management in the prophylactic and therapeutic Read more…

By Nathan Holland, ago

Characterization of a recombinant humanized anti-cocaine monoclonal antibody produced from multiple clones for the selection of a master cell bank candidate.

We have generated a humanized anti-cocaine monoclonal antibody (mAb), which is at a complicated stage of pre-clinical growth. We report right here in vitro binding affinity research, and in vivo pharmacokinetic and efficacy research of the recombinant mAb. The total intention was to characterize the recombinant antibody from every of Read more…

By Nathan Holland, ago
Anti-human Interleukin(IL)-4 Clone 8D4-8 Cross-Reacts With Myosin-9 Associated With Apoptotic Cells and Should Not Be Used for Flow Cytometry Applications Querying IL-4 Expression.

Anti-human Interleukin(IL)-4 Clone 8D4-8 Cross-Reacts With Myosin-9 Associated With Apoptotic Cells and Should Not Be Used for Flow Cytometry Applications Querying IL-4 Expression.

Interleukin(IL)-Four is produced by T cells and different leukocytes and is a crucial mediator of monocyte and B cell responses. During routine circulation cytometry panel validation for the investigation of intracellular cytokines, we noticed distinctive IL-4 expression patterns related to the broadly obtainable monoclonal antibody 8D4-8. Namely, IL-4 (8D4-8) expression Read more…

By Nathan Holland, ago

Clinical validation of the PCR-reverse dot blot human papillomavirus genotyping test in cervical lesions from Chinese women in the Fujian province: a hospital-based population study.

OBJECTIVE To decide the medical significance of the polymerase chain response (PCR)-reverse dot blot (RDB) human papillomavirus (HPV) genotyping assay in cervical most cancers screening. METHODS A complete of 10,442 women attending the Fujian Provincial Maternity and Children’s Health Hospital have been evaluated utilizing the liquid-based cytology (thinprep cytologic test Read more…

By Nathan Holland, ago
PCR-reverse dot blot human papillomavirus genotyping as a primary screening test for cervical cancer in a hospital-based cohort.

PCR-reverse dot blot human papillomavirus genotyping as a primary screening test for cervical cancer in a hospital-based cohort.

To consider the polymerase chain response (PCR)-reverse-dot-blot (RDB) human papillomavirus (HPV) genotyping test as a possible assay for the cervical cancer primary screening.In a hospital-based cohort, a complete of 21,568 ladies have been voluntarily enrolled from March 2009 to November 2016 for evaluating the three present cervical cancer screening methods: Read more…

By Nathan Holland, ago

Probleme aus den Western-Blot-Tests

Der folgende Leitfaden dient als Checkliste für die möglichen Ursachen und Lösungen in Bezug auf einige der am häufigsten auftretenden Probleme aus den Western-Blot-Tests. Hoher Hintergrund S.No. Mögliche Ursache Lösung 1 Zu hohe Antikörperkonzentration Optimieren und verringern Sie die Antikörperkonzentration 2 Aggregierte sekundäre Antikörperbildung Filtriere den sekundären Antikörper durch ein Read more…

By Nathan Holland, ago

Human Genomic Ressources

H.influenzae UniProtKB/Swiss-Prot entries, gene names and WWW servers H.pylori UniProtKB/Swiss-Prot entries, gene names and WWW servers Haemophilia B mutation Database- Factor IX HAGR – The Human Aging Genomic Resources Halobacterium NRC-1 – Halobacterium strain NRC-1 genome project HAMAP  HAMSTeRS – The Haemophilia A Mutation Database and Factor VII Resource site Hardin MD – Hardin meta directory Read more…

By Nathan Holland, ago