Conductance and ion selectivity of a mesoscopic protein nanopore probed with cysteine scanning mutagenesis.

Conductance and ion selectivity of a mesoscopic protein nanopore probed with cysteine scanning mutagenesis.

Nanometer-scale proteinaceous pores are the idea of ion and macromolecular transport in cells and organelles. Recent research recommend that ion channels and artificial nanopores might show helpful in biotechnological purposes. To higher perceive the structure-function relationship of nanopores, we’re finding out the ion-conducting properties of channels fashioned by wild-type and genetically engineered Read more…

A new generation of protein display scaffolds for molecular recognition.

A new generation of protein display scaffolds for molecular recognition.

Engineered antibodies and their fragments are invaluable instruments for an enormous vary of biotechnological and pharmaceutical purposes. However, they’re going through growing competitors from a new generation of protein display scaffolds, particularly chosen for binding just about any goal. Some of them have already entered medical trials. Most of these nonimmunoglobulin proteins Read more…

Bacterial virulence proteins as tools to rewire kinase pathways in yeast and immune cells.

Bacterial virulence proteins as tools to rewire kinase pathways in yeast and immune cells.

Bacterial pathogens have developed particular effector proteins that, by interfacing with host kinase signalling pathways, present a mechanism to evade immune responses throughout an infection. Although these effectors contribute to pathogen virulence, we realized that they may additionally serve as worthwhile artificial biology reagents for engineering mobile behaviour. Here we exploit two Read more…

Vitamin D Forschung

It is believed that prenatal vitamin D deficiency can contribute to the development of wheezing and childhood asthma. In addition to its well-known functions for bone development and health, vitamin D occurs as a pleiotropic hormone that exerts several extrasceletal effects, some of which could reduce the risk of asthma. Read more…

Entschlüsselung der ERBB-Netzwerkdynamik nach Betacellulinsignalisierung beim duktalen Pankreas-Adenokarzinom bei Mäusen.

Das duktale Pankreas-Adenokarzinom (PDAC) wird bald zu den drei häufigsten Krebskillern gehören. Die einzige zugelassene spezifische PDAC-Therapie zielt auf den epidermalen Wachstumsfaktorrezeptor (EGFR) ab. Obwohl EGFR ein entscheidender Akteur bei der PDAC-Entwicklung ist, ist die EGFR-basierte Therapie enttäuschend. In dieser Studie untersuchten wir die Rolle des EGFR-Liganden Betacellulin (BTC) bei Read more…

Cell Biology

Cell Types Primary Cells Immortalized Cells Tumor Cells Drug Discovery Cell Lines Reporter Cell Lines Cell Biology Reagents Media and Supplements Transfection Reagents Mycoplasma Detection and Elimination Cell Attachment Factors Other Cell Culture Reagents Transduction Enhancers Cell Immortalization Kits Cell-based Assay Products Cell Lysates and Total RNA Luciferase Assay Kit Read more…