Bacterial pathogens have developed particular effector proteins that, by interfacing with host kinase signalling pathways, present a mechanism to evade immune responses throughout an infection.

Although these effectors contribute to pathogen virulence, we realized that they may additionally serve as worthwhile artificial biology reagents for engineering mobile behaviour.

Here we exploit two effector proteins, the Shigella flexneri OspF protein and Yersinia pestis YopH protein, to rewire kinase-mediated responses systematically each in yeast and mammalian immune cells.

Yersinia pseudotuberculosis PCR kit

PCR-H190-96D 100T
EUR 572

Yersinia pestis PCR kit

PCR-H604-48D 50T
EUR 453

Yersinia pestis PCR kit

PCR-H604-96D 100T
EUR 572

Yersinia ruckeri PCR kit

PCR-V250-48D 50T
EUR 453

Yersinia ruckeri PCR kit

PCR-V250-96D 100T
EUR 572

Yersinia enterocolitica PCR kit

PCR-VH333-48D 50T
EUR 453

Yersinia enterocolitica PCR kit

PCR-VH333-96D 100T
EUR 572

Yersinia IgG ELISA Kit

DEIA390 96T
EUR 580
Description: The Yersinia IgG Antibody ELISA Test Kit has been designed for the the detection and the quantitative determination of specific IgG antibodies against Yersiniaenterocolitica in serum and plasma.

Yersinia IgA ELISA Kit

DEIA391 96T
EUR 580
Description: The Yersinia IgA Antibody ELISA Test Kit has been designed for the the detection and the quantitative determination of specific IgA antibodies against Yersiniaenterocolitica in serum and plasma.

Yersinia IgM ELISA Kit

DEIA392 96T
EUR 580
Description: The Yersinia IgM Antibody ELISA Test Kit has been designed for the the detection and the quantitative determination of specific IgM antibodies against Yersiniaenterocolitica in serum and plasma.

Yersinia pestis 60KDA chaperonin (groL)

1-CSB-YP394210YAL
  • EUR 679.00
  • EUR 335.00
  • EUR 2172.00
  • EUR 1051.00
  • EUR 1442.00
  • EUR 435.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Yersinia pestis 60KDA chaperonin(groL) expressed in Yeast

Bacterial effector proteins could be directed to inhibit particular mitogen-activated protein kinase pathways selectively in yeast by artificially concentrating on them to pathway-specific complexes.

Moreover, we present that distinctive properties of the effectors generate new pathway behaviours: OspF, which irreversibly inactivates mitogen-activated protein kinases, was used to assemble an artificial suggestions circuit that exhibits novel frequency-dependent enter filtering.

Finally, we present that effectors can be utilized in T cells, both as suggestions modulators to tune the T-cell response amplitude exactly, or as an inducible pause swap that may briefly disable T-cell activation.

Chicken Tcell Growth Factor III (TCGF3) ELISA Kit

abx356219-96tests 96 tests
EUR 825

Monkey Tcell Growth Factor III (TCGF3) ELISA Kit

abx359871-96tests 96 tests
EUR 825

Human Tcell Growth Factor III (TCGF-III) ELISA Kit

abx351529-96tests 96 tests
EUR 707

These research exhibit how pathogens may present a wealthy toolkit of components to engineer cells for therapeutic or biotechnological purposes.

Bacterial virulence proteins as tools to rewire kinase pathways in yeast and immune cells.

Bacterial virulence proteins as tools to rewire kinase pathways in yeast and immune cells.

Potential purposes of enzymes immobilized on/in nano supplies: A evaluate.

Several new varieties of carriers and applied sciences have been carried out in the current previous to enhance conventional enzyme immobilization which aimed to improve enzyme loading, exercise and stability to lower the enzyme biocatalyst price in industrial biotechnology.

These embody cross-linked enzyme aggregates, microwave-assisted immobilization, click on chemistry know-how, mesoporous helps and most just lately nanoparticle-based immobilization of enzymes.

The union of the precise bodily, chemical, optical and electrical properties of nanoparticles with the precise recognition or catalytic properties of biomolecules has led to their look in myriad novel biotechnological purposes.

They have been utilized time and once more for immobilization of industrially necessary enzymes with improved traits.

The excessive surface-to-volume ratio supplied by nanoparticles resulted in the focus of the immobilized entity being significantly greater than that afforded by experimental protocols primarily based on immobilization on planar 2-D surfaces.

Enzymes immobilized on nanoparticles confirmed a broader working pH and temperature vary and greater thermal stability than the native enzymes.

DiagNano Gold Nanoparticle Passive Conjugation Kit, 60 nm

GPK-60 1 kit
EUR 715

DiagNano Gold Nanoparticle Passive Conjugation Kit, 70 nm

GPK-70 1 kit
EUR 715

DiagNano Gold Nanoparticle Passive Conjugation Kit, 80 nm

GPK-80 1 kit
EUR 715

DiagNano Gold Nanoparticle Passive Conjugation Kit, Dot-blot

GPK-DB 1 kit
EUR 814

DiagNano Gold Nanoparticle Passive Conjugation Kit, Lateral Flow

GPK-LF 1 kit
EUR 814

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 100 nm

GCK-M-100 1 kit
EUR 757

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 15 nm

GCK-M-15 1 kit
EUR 757

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 20 nm

GCK-M-20 1 kit
EUR 757

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 30 nm

GCK-M-30 1 kit
EUR 757

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 40 nm

GCK-M-40 1 kit
EUR 757

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 5 nm

GCK-M-5 1 kit
EUR 757

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 50 nm

GCK-M-50 1 kit
EUR 757

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 60 nm

GCK-M-60 1 kit
EUR 757

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 70 nm

GCK-M-70 1 kit
EUR 757

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 90 nm

GCK-M-90 1 kit
EUR 757

Compared with the traditional immobilization strategies, nanoparticle primarily based immobilization served three necessary options; (i) nano-enzyme particles are straightforward to synthesize in excessive strong content material with out utilizing surfactants and poisonous reagents, (ii) homogeneous and nicely outlined core-shell nanoparticles with a thick enzyme shell could be obtained, and (iii) particle measurement could be conveniently tailor-made inside utility limits.

In addition, with the rising consideration paid to cascade enzymatic response and in vitro artificial biology, it’s doable that co-immobilization of multi-enzymes could possibly be achieved on these nanoparticles.

Ketone Body Enzymatic colorimetric micro assay kit, 96 test, Quantitative

1260-KTN-1 1 kit
EUR 408

Glutathione Peroxidase Assay (GPX), Enzymatic colorimetric assay kit, 96 test, Quantitative

1240-GPX-1 1 kit
EUR 590