Bacterial pathogens have developed particular effector proteins that, by interfacing with host kinase signalling pathways, present a mechanism to evade immune responses throughout an infection.

Although these effectors contribute to pathogen virulence, we realized that they may additionally serve as worthwhile artificial biology reagents for engineering mobile behaviour.

Here we exploit two effector proteins, the Shigella flexneri OspF protein and Yersinia pestis YopH protein, to rewire kinase-mediated responses systematically each in yeast and mammalian immune cells.

YERSINIA SELECTIVE AGAR

Y25-100-10kg 10 kg
EUR 1447

YERSINIA SELECTIVE AGAR

Y25-100-2kg 2kg
EUR 354

YERSINIA SELECTIVE AGAR

Y25-100-500g 500 g
EUR 133

Yersinia IgG ELISA Kit

DEIA390 96T
EUR 580
Description: The Yersinia IgG Antibody ELISA Test Kit has been designed for the the detection and the quantitative determination of specific IgG antibodies against Yersiniaenterocolitica in serum and plasma.

Yersinia IgA ELISA Kit

DEIA391 96T
EUR 580
Description: The Yersinia IgA Antibody ELISA Test Kit has been designed for the the detection and the quantitative determination of specific IgA antibodies against Yersiniaenterocolitica in serum and plasma.

Yersinia IgM ELISA Kit

DEIA392 96T
EUR 580
Description: The Yersinia IgM Antibody ELISA Test Kit has been designed for the the detection and the quantitative determination of specific IgM antibodies against Yersiniaenterocolitica in serum and plasma.

Yersinia pseudotuberculosis PCR kit

PCR-H190-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Yersinia pseudotuberculosis

Yersinia pseudotuberculosis PCR kit

PCR-H190-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Yersinia pseudotuberculosis

Yersinia pestis PCR kit

PCR-H604-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Yersinia pestis

Yersinia pestis PCR kit

PCR-H604-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Yersinia pestis

Yersinia ruckeri PCR kit

PCR-V250-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Yersinia ruckeri

Bacterial effector proteins could be directed to inhibit particular mitogen-activated protein kinase pathways selectively in yeast by artificially concentrating on them to pathway-specific complexes.

Moreover, we present that distinctive properties of the effectors generate new pathway behaviours: OspF, which irreversibly inactivates mitogen-activated protein kinases, was used to assemble an artificial suggestions circuit that exhibits novel frequency-dependent enter filtering.

Finally, we present that effectors can be utilized in T cells, both as suggestions modulators to tune the T-cell response amplitude exactly, or as an inducible pause swap that may briefly disable T-cell activation.

Rabbit Tcell Receptor (TCR) ELISA Kit

abx363477-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Guinea pig Tcell Receptor (TCR) ELISA Kit

abx357673-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Monkey Tcell Growth Factor III (TCGF3) ELISA Kit

abx359871-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Pig Tcell Growth Factor III (TCGF3) ELISA Kit

abx361636-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Rabbit Tcell Growth Factor III (TCGF3) ELISA Kit

abx362520-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Chicken Tcell Growth Factor III (TCGF3) ELISA Kit

abx356219-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Human Tcell Growth Factor III (TCGF-III) ELISA Kit

abx351529-96tests 96 tests
EUR 707
  • Shipped within 5-12 working days.

These research exhibit how pathogens may present a wealthy toolkit of components to engineer cells for therapeutic or biotechnological purposes.

Bacterial virulence proteins as tools to rewire kinase pathways in yeast and immune cells.

Bacterial virulence proteins as tools to rewire kinase pathways in yeast and immune cells.

Potential purposes of enzymes immobilized on/in nano supplies: A evaluate.

Several new varieties of carriers and applied sciences have been carried out in the current previous to enhance conventional enzyme immobilization which aimed to improve enzyme loading, exercise and stability to lower the enzyme biocatalyst price in industrial biotechnology.

These embody cross-linked enzyme aggregates, microwave-assisted immobilization, click on chemistry know-how, mesoporous helps and most just lately nanoparticle-based immobilization of enzymes.

The union of the precise bodily, chemical, optical and electrical properties of nanoparticles with the precise recognition or catalytic properties of biomolecules has led to their look in myriad novel biotechnological purposes.

They have been utilized time and once more for immobilization of industrially necessary enzymes with improved traits.

The excessive surface-to-volume ratio supplied by nanoparticles resulted in the focus of the immobilized entity being significantly greater than that afforded by experimental protocols primarily based on immobilization on planar 2-D surfaces.

Enzymes immobilized on nanoparticles confirmed a broader working pH and temperature vary and greater thermal stability than the native enzymes.

DiagNano Gold Nanoparticle Passive Conjugation Kit, 30 nm

GPK-30 1 kit
EUR 715

DiagNano Gold Nanoparticle Passive Conjugation Kit, 40 nm

GPK-40 1 kit
EUR 715

DiagNano Gold Nanoparticle Passive Conjugation Kit, 5 nm

GPK-5 1 kit
EUR 715

DiagNano Gold Nanoparticle Passive Conjugation Kit, 50 nm

GPK-50 1 kit
EUR 715

DiagNano Gold Nanoparticle Passive Conjugation Kit, 60 nm

GPK-60 1 kit
EUR 715

DiagNano Gold Nanoparticle Passive Conjugation Kit, 70 nm

GPK-70 1 kit
EUR 715

DiagNano Gold Nanoparticle Passive Conjugation Kit, 80 nm

GPK-80 1 kit
EUR 715

DiagNano Gold Nanoparticle Passive Conjugation Kit, Dot-blot

GPK-DB 1 kit
EUR 814

DiagNano Gold Nanoparticle Passive Conjugation Kit, Lateral Flow

GPK-LF 1 kit
EUR 814

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 100 nm

GCK-M-100 1 kit
EUR 757

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 15 nm

GCK-M-15 1 kit
EUR 757

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 20 nm

GCK-M-20 1 kit
EUR 757

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 30 nm

GCK-M-30 1 kit
EUR 757

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 40 nm

GCK-M-40 1 kit
EUR 757

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 5 nm

GCK-M-5 1 kit
EUR 757

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 50 nm

GCK-M-50 1 kit
EUR 757

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 60 nm

GCK-M-60 1 kit
EUR 757

Compared with the traditional immobilization strategies, nanoparticle primarily based immobilization served three necessary options; (i) nano-enzyme particles are straightforward to synthesize in excessive strong content material with out utilizing surfactants and poisonous reagents, (ii) homogeneous and nicely outlined core-shell nanoparticles with a thick enzyme shell could be obtained, and (iii) particle measurement could be conveniently tailor-made inside utility limits.

In addition, with the rising consideration paid to cascade enzymatic response and in vitro artificial biology, it’s doable that co-immobilization of multi-enzymes could possibly be achieved on these nanoparticles.

Tryptone (Casein hydrolysate, enzymatic digest)

GE6771-250G 250 g
EUR 75

Tryptone (Casein hydrolysate, enzymatic digest)

GE6771-500G 500 g
EUR 110

3D Cell Culture Non-Enzymatic Cell Harvesting Kit

K982-100
EUR 272

Glutathione (GSH) Assay, Enzymatic colorimetric assay kit, 96 test, Quantitative

1250-GSH-1 1 kit
EUR 408

Ketone Body Enzymatic colorimetric micro assay kit, 96 test, Quantitative

1260-KTN-1 1 kit
EUR 408

Glutathione Peroxidase Assay (GPX), Enzymatic colorimetric assay kit, 96 test, Quantitative

1240-GPX-1 1 kit
EUR 590