In June 2020, JOYSBIO Biotechnology proudly launched a brand new COVID-19 Antigen Rapid Test Kit (Colloidal Gold). The new coronavirus antigen check package is a lateral stream immunoassay for the qualitative detection of SARS-COV-2 antigen (nucleocapsid protein) in higher respiratory samples with nasal swabs or saliva through the acute part of an infection. An uncut sheet format is offered.

Features

  • 15-minute fast detection
  • Easy-to-operate coronavirus antigen check
  • Less-invasive nasal (NS) swab pattern assortment
  • CE-IVD marked
  • Available in 1/2/5/20 checks/field

Performance Characteristics

JOYSBIO’s coronavirus Ag check package was independently evaluated at Centro Diagnostico Delta S.r.l. in Italy between October 2020 and January 2021. A complete of 107 optimistic specimens have been examined with JOYSBIO’s COVID-19 Antigen Rapid Test Kit. These specimens have been collected from sufferers who’re suspected of COVID-19 with nasal swabs. The coronavirus antigen check package’s sensitivity and specificity are in contrast towards a CE-IVD marked RT-PCR check package. This scientific analysis is performed underneath the idea that SARS-CoV is now not spreading locally. 

According to the scientific evaluation of 492 samples, the detection sensitivity is 98.13%, and the specificity is 99.22%.

  • Positive Percent Agreement (PPA) = 105/107 (98.13%) (95%CI: 93.4%~99.8%)
  • Negative Percent Agreement (NPA) = 382/385 (99.22%) (95%CI:97.7%~99.8%)
  • Accuracy = (105+382)/492×100%=98.98%
  • Kappa = 2×(105×382-3×2)/(108×385+107 ×384) = 0.97>0.5

The restrict of detection (LOD) of this product is 1.6 x 102 TCID50/mL, calculated by means of a gradient dilution methodology.

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imuno ace 2

COVID-19 Antigen Rapid Test Principle

The coronavirus antigen fast check package is a lateral stream assay that qualitatively detects the presence of nucleocapsid (N) protein in higher respiratory samples (nasal swabs). This lateral stream assay is designed with the sandwich immunoassay format. When the specimen is added onto the pattern pad of a check cassette, coronavirus N protein binds with colloidal gold-labeled SARS-CoV-2 N protein antibody to kind an antibody-antigen (Ab-Ag) advanced. The Ab-Ag advanced is captured by SARS-CoV-2 N protein antibody (Rabbit monoclonal antibody) when migrating to the check line underneath capillary motion. A red-colored band will seem on the check line, which signifies the specimen is COVID-19 nucleocapsid protein optimistic. No shade band will seem on the check line if the specimen doesn’t comprise any coronavirus antigen (N protein), or the antigen stage is under detection restrict.

  1. Twist off the cap of the buffer bottle, fastidiously dispense all buffer into the extraction tube。 
  2. After accumulating higher respiratory pattern with nasal swab, insert the swab into the extraction tube, plunge the swab up and down within the fluid for at least 10 seconds. Hold the swab towards the underside of the tube, rotate three turns. DO NOT splash liquid out of the tube. 
  3. Remove the swab whereas squeezing the perimeters of the tube to extract the liquid from the swab. 
  4. Press the nozzle cap firmly onto the extraction tube. Mix totally by swirling or flicking the underside of the tube. 
  5. Gently squeeze the tube’s inflexible physique, dispense two (2) drops of the buffer-specimen combination into the pattern properly on the coronavirus antigen check cassette.
  6. Read the check outcomes between 15 and 20 minutes. Do not learn the outcomes after 20 minutes.

Stage 1: Nasopharyngeal swab specimen assortment:
1. Collect a nasopharyngeal swab specimen by inserting the sterile swab into the nostril.
2. Push the sterile swab till resistance is met on the stage of the turbinate.
3. Rotate the sterile swab a number of instances towards the nasopharyngeal wall & depart within the place for 10 seconds to saturate the swab tip.
4. Remove the swab from the nostril fastidiously.
5. Place the swab specimen into the viral transport medium buffer tube and shut the tube tightly.
6. Transport the swab pattern in VTM to the laboratory in chilly chain.
7. The pattern will be saved within the Room temperature (Below 30◦C) as much as 24 hrs from the time of pattern assortment or at 2 – 8◦C for as much as 48 hrs from the time of pattern assortment.

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imuno ace

Stage 2: Sample preparation for testing:
1. Sample preparation must be carried out in BSL-2 stage cupboard within the Laboratory.
2. Mix the swab specimen in VTM tube properly (vortex roughly 3-5 seconds).
3. Transfer 100 μL VITROS® SARS-CoV-2 Antigen Extraction Buffer right into a labelled new pattern tube.

4. Add 400 μL viral pattern to the above tube (to take care of 1:Four ratio of extraction buffer: pattern)
5. Mix properly (Cap/Plug the pattern tube and vortex roughly 3-5 seconds)
Stage 3: Sample processing in VITROS® methods:
1. Place/load the ready/extracted pattern tube in stage 2, after de-capping on to the VITROS® instrument V3600/V5600/XT7600; An quantity of 80 μL of extracted pattern is used for every willpower.
2. Program VITROS 3600 / VITROS 5600 / VITROS XT 7600 system to course of the samples for CV2Ag. The system can used to program check both in a STAT/Random/Batch mode.
3. System processes the samples routinely utilizing disposable VersaTips for each pattern in addition to reagents to stop any cross-contamination. The outcomes shall be delivered in 48 minutes after pattern aspiration, within the type of S/Co worth.

Human Streptococcus Pneumoniae (SP) Antigen Rapid Test Kit

abx092096-20tests 20 tests
EUR 398
  • Shipped within 5-12 working days.

Avian Influenza Virus Antigen Rapid Test Kit (Colloidal gold)

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EUR 432
  • Shipped within 5-12 working days.

Newcastle Disease Virus Antigen Rapid Test Kit (Colloidal gold)

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  • Shipped within 5-12 working days.

Human Chlamydia Trachomatis Antigen Rapid Test Kit (Colloidal gold)

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EUR 230
  • Shipped within 5-12 working days.

Melamine (MEL) Rapid Test Kit

abx092057-50tests 50 tests
EUR 370
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Clenbuterol (CLE) Rapid Test Kit

abx092058-50tests 50 tests
EUR 244
  • Shipped within 5-12 working days.

Ractopamine (RAC) Rapid Test Kit

abx092059-50tests 50 tests
EUR 244
  • Shipped within 5-12 working days.

Salbutamol (SAL) Rapid Test Kit

abx092060-50tests 50 tests
EUR 244
  • Shipped within 5-12 working days.

Tetracycline (TCs) Rapid Test Kit

abx092063-50tests 50 tests
EUR 398
  • Shipped within 5-12 working days.

Sulfonamides (Sas) Rapid Test Kit

abx092064-40tests 40 tests
EUR 398
  • Shipped within 5-12 working days.

Quinolones (QNs) Rapid Test Kit

abx092065-40tests 40 tests
EUR 398
  • Shipped within 5-12 working days.

Ciprofloxacin (CPFX) Rapid Test Kit

abx092066-50tests 50 tests
EUR 398
  • Shipped within 5-12 working days.

Quinolones (QNs) Rapid Test Kit

abx092067-40tests 40 tests
EUR 398
  • Shipped within 5-12 working days.

Brucella Antibody Rapid Test Kit

abx092069-40tests 40 tests
EUR 356
  • Shipped within 5-12 working days.

Rapid PED Ag Test Kit

RG14-01 1 box
EUR 159.9
Description: Please check the datasheet of Rapid PED Ag Test Kit before using the test.

Rapid Leishmania Ab Test Kit

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EUR 127
Description: Please check the datasheet of Rapid Leishmania Ab Test Kit before using the test.

IBD rapid test strip

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EUR 148
Description: Please check the datasheet of IBD rapid test strip before using the test.

Rabies rapid test strip

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EUR 151.92
Description: Please check the datasheet of Rabies rapid test strip before using the test.

Accu-Tell COVID-19 IgG/IgM Rapid Test

GEN-B352-20tests 20 tests
EUR 236
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Accu-Tell COVID-19 IgG/IgM Rapid Test

GEN-B352-40tests 40 tests
EUR 321
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Avian Influenza H5 Virus Antigen Rapid Test Kit (Colloidal gold)

abx092014-40tests 40 tests
EUR 488
  • Shipped within 5-12 working days.

Avian Influenza H7 Virus Antigen Rapid Test Kit (Colloidal gold)

abx092017-40tests 40 tests
EUR 432
  • Shipped within 5-12 working days.

Clenbuterol Rapid Test Kit (Colloidal gold)

abx092040-50tests 50 tests
EUR 272
  • Shipped within 5-12 working days.

Ractopamine Rapid Test Kit (Colloidal gold)

abx092042-50tests 50 tests
EUR 300
  • Shipped within 5-12 working days.

Salbutamol Rapid Test Kit (Colloidal gold)

abx092043-50tests 50 tests
EUR 342
  • Shipped within 5-12 working days.

Chloramphenicol Rapid Test Kit (Colloidal gold)

abx092045-50tests 50 tests
EUR 356
  • Shipped within 5-12 working days.

Quinolones Rapid Test Kit (Colloidal gold)

abx092046-50tests 50 tests
EUR 537
  • Shipped within 5-12 working days.

Sulfonamides Rapid Test Kit (Colloidal gold)

abx092047-50tests 50 tests
EUR 551
  • Shipped within 5-12 working days.