Abstract

Nanocomposite double-network hydrogels (ncDN hydrogels) are lately launched to handle the constraints of conventional DN hydrogels, corresponding to the dearth of range within the community construction and the restricted functionalities. However, two challenges stay, together with the time-consuming preparation and the dearth of shear-thinning and self-healing properties. Here, our method to creating versatile ncDN hydrogels is thru using a number of interfacial crosslinking chemistries (i.e., noncovalent interactions of electrostatic interplay and hydrogen bonds in addition to dynamic covalent interactions of imine bonds and boronate ester bonds) and floor functionalized nanomaterials (i.e. phenylboronic acid modified diminished graphene oxide (PBA-rGO)). PBA-rGO was used as a multivalent gelator to additional crosslink the 2 polymer chains (i.e. triethylene glycol-grafted chitosan (TEG-CS) and polydextran aldehyde (PDA)) in DN hydrogels, forming the TEG-CS/PDA/PBA-rGO ncDN hydrogels in seconds. The microstructures (i.e. pore dimension) and properties (i.e. rheological, mechanical, and swelling properties) of the ncDN hydrogels could be merely modulated by altering the quantity of PBA-rGO. The dynamic bonds within the polymeric community offered the shear-thinning and self-healing properties to the ncDN hydrogels, permitting the hydrogels to be injected and molded into different shapes in addition to self-repair the broken construction. Besides, the designed TEG-CS/PDA/PBA-rGO ncDN hydrogels had been cytocompatible and in addition exhibited antibacterial exercise. Taken collectively, we hereby present a nanomaterial method to manufacture a brand new class of ncDN hydrogels with tailorable networks and favourite properties for particular functions.

General description

Agarose is obtained from seaweed and incorporates repeated agarobiose items. During agarose gel formation, the polymers combination to type a community with various pore sizes. This property is utilized in agarose gel electrophoresis to separate DNA.

Application

  • Agarose LE  is very suited  for analyzing fragments between 0.2 and 15 kb in: Analysis of PCR merchandise
  • Examination of restriction endonucleases
  • Digests of plasmid, cosmid, and λ phage DNA
  • Electrophoresis of RNA in, e.g., denaturing gels containing formaldehyde

Nucleic acid fragments separated with Agarose LE could be blotted to nylon or nitro-cellulose membranes by all commonplace blotting strategies.
Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, doesn’t intrude with using Agarose LE.

Quality

Absence of DNase: none detected in keeping with the present Quality Control procedures.
Absence of RNase: none detected in keeping with the present Quality Control procedures.

Other Notes

For life science analysis solely. Not to be used in diagnostic procedures.
Agarose LE

Agarose LE

PROPERTIES

type

powder

packaging

pkg of 100 g (11685660001)
pkg of 500 g (11685678001)

producer/tradename

Roche

shipped in

ambient

storage temp.

20-25°C

Agarose LE

A-1270-500 500 g
EUR 291

Benchmark Agarose LE

A1700 1 PC
EUR 321.65
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Benchmark Agarose LE

A1701 1 PC
EUR 100.09
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Benchmark Agarose LE

A1705 1 PC
EUR 150.33
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Agarose GPG/LE

A2500-100000 100 g
EUR 142
Description: Agar is a galactan polysaccharides which extracted from the intracellular matrix in marine algae Rhodophyta. Agarose is a neutral polymer component of agar. Agarose solution transforms into gel after heating. Gel is formed resulting from double helical formation from molecular chains.

Agarose GPG/LE

A2500-500000 500 g
EUR 467
Description: Agar is a galactan polysaccharides which extracted from the intracellular matrix in marine algae Rhodophyta. Agarose is a neutral polymer component of agar. Agarose solution transforms into gel after heating. Gel is formed resulting from double helical formation from molecular chains.

GelRed Agarose LE

41029-50G 50G
EUR 320
Description: Minimum order quantity: 1 unit of 50G

GelRed Agarose LE

41029-5G 5G
EUR 104
Description: Minimum order quantity: 1 unit of 5G

GelGreen Agarose LE

41030-50G 50G
EUR 320
Description: Minimum order quantity: 1 unit of 50G

GelGreen Agarose LE

41030-5G 5G
EUR 104
Description: Minimum order quantity: 1 unit of 5G

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT

AGR-LE-100 1/pk
EUR 168
Description: Bioscience Mol Bio; Agarose

Sepro Agarose LE Tablet

A0250-025 25 Tabs (12.5g)
EUR 103

Sepro Agarose LE Tablet

A0250-050 500 Tabs (250g)
EUR 388

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 500G/UNIT, 10 UNITS PER CASE

AGR-LE-5001 1/pk
EUR 3058
Description: Bioscience Mol Bio; Agarose

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT, 10 UNITS PER CASE

AGR-LE-1001 1/pk
EUR 772
Description: Bioscience Mol Bio; Agarose

HyAgarose? LE Agarose, Multi-purpose, 100g

R9012LE-100g
EUR 163

HyAgarose? LE Agarose, Multi-purpose, 500g

R9012LE-500g
EUR 417

Agarose LE, Ultra-Pure Molecular Biology Grade, 100 g

41028-100G 100G
EUR 222
Description: Minimum order quantity: 1 unit of 100G

Agarose LE, Ultra-Pure Molecular Biology Grade, 25 g

41028-25G 25G
EUR 109
Description: Minimum order quantity: 1 unit of 25G

Agarose LE, Ultra-Pure Molecular Biology Grade, 500 g

41028-500G 500G
EUR 719
Description: Minimum order quantity: 1 unit of 500G

LE 300

B6824-10 10 mg
EUR 383

LE 135

B6928-10 10 mg
EUR 206

LE 135

B6928-5 5 mg
EUR 163

25G AquaPor LE

NAT1020 25G
EUR 110

Agarose

G060-1 100 g
EUR 176

Agarose

G060-2 500 g
EUR 369

Agarose

abx082006-100g 100 g
EUR 244
  • Shipped within 5-10 working days.

Agarose

abx082007-100g 100 g
EUR 258
  • Shipped within 5-10 working days.