Phire and Phusion Kits

What is Direct PCR?

Direct PCR (dPCR) is a technique of DNA amplification instantly from an animal or plant tissue pattern with out performing DNA isolation and purification steps. This approach vastly reduces experimental time, and price in genotyping and high-volume initiatives. It additionally offers a greater possibility when confronted with the challenges of amplifying a really small amount of pattern the place the purification step might doubtlessly result in pattern loss.

In some ways, dPCR works like typical PCR. Both methods rely upon template DNA, primers and a grasp combine or PCR reagents, and each use a thermal cycler for amplification. The major distinction between dPCR and typical PCR is the tailor-made buffers utilized in dPCR. The modified parts of dPCR allow strong amplification regardless of the presence of PCR inhibitors typically present in crude samples.

ALS SARS-CoV-2 RT-PCR 4G has been developed and produced fully at ALS in Portugal and is designed primarily for export. This new equipment is ready to detect all identified SARS-CoV-2 variants, together with British, Brazilian and South African variants, with out cross-reacting with different identified associated viruses or frequent respiratory pathogens. The alternative of the genomic areas used as targets and the sequences of the primers and hydrolysis probes had been primarily based on WHO, CDC and DGS suggestions.

The ALS SARS-CoV-2 RT-PCR 4G equipment is an in vitro diagnostic medical gadget (CE-IVD) that makes use of real-time PCR nucleic acid amplification know-how for the detection of SARS-CoV-2 in medical samples from the higher respiratory tract of people who might or is probably not suspected of getting COVID-19.

The equipment makes use of the RT-PCR approach, combining amplification of sure areas of the viral genome with its detection by particular hydrolysis probes. The SARS-CoV-2 genome goal areas are extremely conserved and correspond to 2 particular genes: ORF polyprotein (ORF1ab gene) and nucleocapsid phosphoprotein (N gene). The equipment may detect the gene that codifies the structural protein of the envelope (gene E) particular to the Sarbecovirus subgenus.

Advantages

• Rapid amplification: The amplification price of the polymerase is 6kb/min. 1kb fragment might be amplified inside 25min.
• Long fragment amplification: For plasmid, λ DNA and different straightforward templates, the polymerase can successfully amplify > 20kb. For genome, the polymerase can successfully amplify > 8kb. And for cDNA, the polymerase can successfully amplify > 8kb.
• High specificity: With sizzling begin know-how, the polymerase is 100% inactive under 50°C, and can solely be restored by heating at 95°C for 5min.
• High tolerance to impurities: Samples of entire blood, serum, cultured cells and urine might be instantly amplified with out prior DNA purification.

Unit Definition

One unit is outlined as the quantity of enzyme required to catalyze the incorporation of 10 nmol dNTP into an acid insoluble substance inside 30 min at 74°C.

Description	Code	Specification	Category	Technology
ALS SARS-CoV-2 RT-PCR Test for surfaces	ALS-B01-Adv	RUO	Food /Water Virology Detection	Real-Time PCR
ALS SARS-CoV-2 RT-PCR Test for Wastewater	ALS-B02-Adv	RUO	Food /Water Virology Detection	Real-Time PCR

 

 

Plant Direct PCR Kit
PD105-01	Vazyme	50 rxn (50 μl/rxn)	EUR 169

Plant Direct PCR Kit
PD105-02	Vazyme	200 rxn (50 μl/rxn)	EUR 354

amfiSure Direct PCR Lysis Buffer
D0300-010	GenDepot	100ml	EUR 138

Blood Direct PCR Kit V2
PD103-01	Vazyme	50 rxn	EUR 169

Blood Direct PCR Kit V2
PD103-02	Vazyme	200 rxn	EUR 354

Mouse Direct PCR Kit (For Genotyping)
B40013	Bimake	200 ractions	EUR 209
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)
B40015	Bimake	500 ractions	EUR 409
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)
B45012	Bimake	1ml*5 (500 ractions)	EUR 280
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)
B45013	Bimake	1ml*20 (2000 ractions)	EUR 865
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Direct RT-PCR Kit wioRealStart DNA Polymerase
FYT507-100P	Yeastern Biotech	100 Preps	Ask for price

Direct RT-PCR Kit wioRealStart DNA Polymerase
FYT507-50P	Yeastern Biotech	50 Preps	Ask for price

FastAmp Plant Direct PCR Kit - 250 Reactions
4612	Intact Genomics	1/EA	EUR 164

Direct RT-PCR Kit wi RealStart DNA Polymerase
FYT506-100P	Yeastern Biotech	100 Preps	Ask for price

Direct RT-PCR Kit wi RealStart DNA Polymerase
FYT506-50P	Yeastern Biotech	50 Preps;	Ask for price

Allele-In-One Human Blood Direct PCR Kit
ABP-PP-BD02100	Allele Biotech	100 RXNS	Ask for price

Allele-In-One Human Blood Direct PCR Kit
ABP-PP-BD02500	Allele Biotech	500 RXNS	Ask for price

Allele-In-One Mouse Tail Direct PCR Kit
ABP-PP-MT02100	Allele Biotech	100 RXNS	Ask for price

Allele-In-One Mouse Tail Direct PCR Kit
ABP-PP-MT02500	Allele Biotech	500 RXNS	Ask for price

MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-48D	Bioingentech	50T	EUR 453

MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-96D	Bioingentech	100T	EUR 572

MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-48D	Bioingentech	50T	EUR 453

MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-96D	Bioingentech	100T	EUR 572

Halobacillus PCR kit
PCR-A635-48D	Bioingentech	50T	EUR 453

Halobacillus PCR kit
PCR-A635-96D	Bioingentech	100T	EUR 572

Enterovirus PCR kit
PCR-H433-48R	Bioingentech	50T	EUR 823

Enterovirus PCR kit
PCR-H433-96R	Bioingentech	100T	EUR 859

Mengovirus PCR kit
PCR-H453-48R	Bioingentech	50T	EUR 823

Mengovirus PCR kit
PCR-H453-96R	Bioingentech	100T	EUR 1113