The preferred and fixed embedding medium for cryotomy

Tissue-Tek O.C.T. Compound is used to shortly embed modern tissue specimens for frozen sections using a cryostat. Tissue-Tek O.C.T. Compound is a formulation of clear, water-soluble glycols and resins, providing a secure matrix to encapsulate tissue specimens and bond to the Specimen Holder for fixed sectioning in a cryostat working temperature of -10°C and beneath.

Undesirable background staining is eradicated as no residue is left on the slides all through staining.

O.C.T stands for optimum chopping temperature. The extreme viscosity of Tissue-Tek O.C.T Compound ends in fast freezing for optimum half prime quality.

The OCT Compound is purchased in 4 oz (118 mL) squeeze bottles; 12/case.

What is exactly in TissueTek OCT compound and what’s its affect on enzyme train of tissues?

Hello all, I’m working with cryo pores and pores and skin samples and I’m making an attempt to do in situ zymography (visualize enzyme acitivity with substrate which turns into fluorescent). The pores and pores and skin samples are prepared with TissueTek, then snap-freezed in liquid nitrogen after which decrease using a cryostat. Nevertheless, I cannot uncover what’s exactly in TissueTek, as a result of it may very well be important clarification for the affect on enzyme train (pH?). Does anyone know if embedding tissues with TissueTek may impact enzyme train in tissues?
tissue-tek-paraform

tissue-tek-paraform

Options and benefits of the Tissue-Tek OCT embedding compound are:

  •   Water soluable mixture of glycols and resins give a gentle embedding matrix
  •   Superior half prime quality at cryostat temperatures from -10°C to -40°C
  •   Extremely soluable in water, alcohol and formalin
  •  Good sectioning properties with disposable cryostat blades and secure knives
  •   Doesn’t go away residues on slides all through staining procedures, eliminating undesirable background staining
  •   Tissue, mounted in OCT compound will probably be frozen between -10°C and -80°C
  •   An glorious mounting medium for cryo-SEM, cryo-FIB/SEM and totally different chilly phases
Specs for this merchandise

tissue-tek

tissue-tek

Product particulars

  • Is Discontinued By Producer ‏ : ‎ No
  • Package deal Dimensions ‏ : ‎ 19.three x 5.three x 4 cm; 113.4 Grams
  • Date First Accessible ‏ : ‎ 13 January 2020
  • Producer ‏ : ‎ Electron Microscopy Sciences
  • ASIN ‏ : ‎ B00Okay33LEBS
  • Merchandise model amount ‏ : ‎ 62550-01
  • Producer ‏ : ‎ Electron Microscopy Sciences
  • Merchandise Weight ‏ : ‎ 113 g
  • Web Amount ‏ : ‎ 1 Answer

The Tissue-Tek optimum chopping temperature (O.C.T.) decision consists of water-soluble glycols and resins that give a specimen matrix for cryostat sectioning at temperatures of –10°C (14°F) and beneath. This product does not go away any residue on slides all through and after staining course of. It’s packaged and acquired individually in a 118mL (4oz.) bottle.

 

Tissue-Tek

abx082403-118ml 118 ml
EUR 230
  • Shipped within 5-10 working days.

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