Total RNA Purification Kits

The purification methods of our Total RNA Purification Kits range from organic extraction reagents all the way to spin columns and magnetic beads.

Starting with high quality, pure, and intact total RNA is critical to many experiments (e.g. RT-PCR, qRT-PCR, array analysis, Northern blots, nuclease protection assays, and RNA-sequencing). The table below will help you choose the right Invitrogen product to purify total RNA from your specific sample type and sample size.

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.

RNA purification kit

Highlights

• Universal – can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
• Efficient – high yields of total RNA.
• Fast – 15 minutes after lysis step
• Pure – an A260/280 ratio > 1.9
• Convenient – spin columns are capped and assembled with collection tubes

Applications

• Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assayIncludes Proteinase K Lysis Buffer Wash Buffer 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with Collection Tubes) Collection Tubes (2 ml) Collection Tubes (1.5 ml) Detailed Protocol

RNA purification

General description

Sigma′s GenElute^™ Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, plasma, serum, bacteria, yeast, fungi, plants and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

• Isolate true total RNA including siRNA and microRNA
• Rapidly remove contaminating genomic DNA without the use of enzymes
• No phenol or chloroform extractions
• Purify high-quality RNA in 20 minutes
• Extract RNA from as little as a single cell
• Isolate from a wide variety of specimens

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