Consists of a single peak for optical alignment of any fl ow cytometer in all channels from UV to Far Red
• Determines if the fl ow cell is clear and with out fl uidic blockage
• Measures the coeffi cients of variation (CVs), peak channels, and histogram distribution to find out
the performance of fl ow cytometers.

New move cytometers with growing variety of fluorescent channels within the UV and Far Red and the provision of corresponding fluorescent conjugates at the moment are accessible. In order to maintain up, we’ve developed the Ultra Rainbow Fluorescent Particles with enhanced UV and Far Red fluorescence depth. The Ultra Rainbow
Fluorescent Particles comprise a single peak and are designed for checking the optical alignment of any move
cytometer in all channels.

 

Description

This product accommodates a single inhabitants of Rainbow Particles which can be dyed to a single fluorescent depth. Every Rainbow Particle accommodates a mix of fluorophores which can be stably embedded inside the particle and might be excited at any wavelength from 365-650 nm, permitting most channels in a move cytometer to be calibrated utilizing the identical set of particles. The Rainbow Particles have emission spectra appropriate with many widespread fluorophores used for immunofluorescent staining by move cytometric evaluation.  Rainbow Fluorescent Particles are supplied in a dropper bottle for handy meting out and storage.

These Rainbow Calibration Particles are a mix of three.0 – 3.four μm particles, dyed to 6 completely different fluorescent intensities. As their excitation vary is 365 – 650 nm, they can be utilized with most lasers. However, they aren’t to be used with the UV laser. The emission spectra of those rainbow particles is appropriate with many widespread fluorophores utilized in immunofluorescent staining with move cytometric evaluation.

Recommended Assay Procedures

This particle combination (~10×10^6 particles/mL) is helpful for routine calibration of move cytometers. Before use, resuspend the particles by vortexing. Dilution of 3-5 drops of particles to 1 ml of sheath fluid will present an ample variety of particles for move cytometric evaluation.

spherotech

spherotech

Product Notices

  1. Caution: Sodium azide yields extremely poisonous hydrazoic acid beneath acidic situations. Dilute azide compounds in operating water earlier than discarding to keep away from accumulation of probably explosive deposits in plumbing.
  2. Cy is a trademark of GE Healthcare.

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