The preferred and fixed embedding medium for cryotomy
Tissue-Tek O.C.T. Compound is used to shortly embed modern tissue specimens for frozen sections using a cryostat. Tissue-Tek O.C.T. Compound is a formulation of clear, water-soluble glycols and resins, providing a secure matrix to encapsulate tissue specimens and bond to the Specimen Holder for fixed sectioning in a cryostat working temperature of -10°C and beneath.
Undesirable background staining is eradicated as no residue is left on the slides all through staining.
O.C.T stands for optimum chopping temperature. The extreme viscosity of Tissue-Tek O.C.T Compound ends in fast freezing for optimum half prime quality.
The OCT Compound is purchased in 4 oz (118 mL) squeeze bottles; 12/case.
What is exactly in TissueTek OCT compound and what’s its affect on enzyme train of tissues?
Options and benefits of the Tissue-Tek OCT embedding compound are:
- Water soluable mixture of glycols and resins give a gentle embedding matrix
- Superior half prime quality at cryostat temperatures from -10°C to -40°C
- Extremely soluable in water, alcohol and formalin
- Good sectioning properties with disposable cryostat blades and secure knives
- Doesn’t go away residues on slides all through staining procedures, eliminating undesirable background staining
- Tissue, mounted in OCT compound will probably be frozen between -10°C and -80°C
- An glorious mounting medium for cryo-SEM, cryo-FIB/SEM and totally different chilly phases
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