Preparation of a novel antiserum to aromatase with high affinity and specificity: Its clinicopathological significance on breast cancer tissue.

Aromatase inhibitors have been broadly used for the endocrine therapy of estrogen-dependent breast cancer in postmenopausal sufferers. However, clinicopathological research of aromatase have been restricted due to unsatisfactory specificity and/or restricted availability of anti-aromatase antibodies. Here, we have now generated a polyclonal antiserum with high affinity and specificity for human aromatase utilizing a monoclonal antibody … Read more

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A Novel Antiserum Against a Predicted Human Peripheral Choline Acetyltransferase (hpChAT) for Labeling Neuronal Structures in Human Colon.

Choline acetyltransferase (ChAT), the enzyme synthesizing acetylcholine (ACh), has an exon-skipping splice variant which is expressed preferentially in the peripheral nervous system (PNS) and thus termed peripheral ChAT (pChAT). A rabbit antiserum beforehand produced towards rat pChAT (rpChAT) has been used for immunohistochemistry (IHC) to research peripheral cholinergic buildings in numerous animals. The current research … Read more

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