Product Description

The BrdU Cell Proliferation Assay Package detects 5-bromo-2’-deoxyuridine (BrdU) integrated into mobile DNA throughout cell proliferation utilizing an anti-BrdU antibody. When cells are cultured with labeling medium that incorporates BrdU, this pyrimidine analog is integrated instead of thymidine into the newly synthesized DNA of proliferating cells.

After eradicating labeling medium, cells are fastened and the DNA is denatured with our fixing/denaturing answer. Then a BrdU mouse mAb is added to detect the integrated BrdU (The denaturing of DNA is critical to enhance the accessibility of the integrated BrdU to the detection antibody). Anti-mouse IgG, HRP-linked antibody is then used to acknowledge the certain detection antibody. HRP substrate TMB is added to develop colour. The magnitude of the absorbance for the developed colour is proportional to the amount of BrdU integrated into cells, which is a direct indication of cell proliferation.

Product Consists of Amount (with Rely) Answer Colour
BrdU 1 x 150 µl
Fixing/denaturing Answer 2 x 25 ml
BrdU Mouse Detection mAb 1 x 500 µl Inexperienced
Anti-mouse IgG, HRP-Linked Antibody 1 x 500 µl Pink
Detection Antibody Diluent 1 x 50 ml Inexperienced
HRP Diluent 1 x 50 ml Pink
20X Wash Buffer 1 x 50 ml
TMB Substrate 7004 1 x 50 ml
STOP Answer 7002 2 x 25 ml
Brdu Cell

Brdu Cell

Background

Halogenated nucleotides such because the pyrimidine analog bromodeoxyuridine (BrdU) are helpful for labeling nascent DNA in residing cells and tissues. BrdU turns into integrated into replicating DNA instead of thymidine and subsequent immunodetection of BrdU utilizing particular monoclonal antibodies permits labeling of cells in S part of the cell cycle. After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb can be utilized to detect BrdU integrated into single stranded DNA.

Key options and particulars

  • Sensitivity: 40 cells/effectively
  • Pattern kind: Adherent cells, Suspension cells
  • Detection technique: Colorimetric
  • Assay kind: Sandwich (qualitative)

5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It will get integrated into the newly synthesized DNA of proliferating cells instead of thymidine. Biovision’s BrdU Cell Proliferation Assay Package detects integrated BrdU utilizing a mouse anti-BrdU antibody. An anti-mouse HRP-linked secondary antibody is used to detect the anti-BrdU antibody certain to BrdU, which is adopted by addition of TMB (a HRP substrate). The extent of colour improvement is proportional to the amount of BrdU integrated into the cells and can be utilized straight as an indicator of cell proliferation. In comparison with different cell proliferation assays, this package detects solely the proliferating cells and never the seeded cells. This extremely delicate, non-radioactive package detects as much less as 50-100 proliferating cells.

METABOLIC PROLIFERATION ASSAYS

Assays that measure metabolic exercise are appropriate for analyzing proliferation, viability, and cytotoxicity. The discount of tetrazolium salts similar to MTT, XTT, and WST-1 to coloured formazan compounds or the bioreduction of resazurin happens solely in metabolically energetic cells. Actively proliferating cells enhance their metabolic exercise, whereas cells uncovered to toxins may have decreased exercise.

 

BrdU Cell Proliferation ELISA Kit (colorimetric)

BrdU Cell Proliferation ELISA Kit (colorimetric)

MTT Cell Proliferation Assays

MTT (3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide; thiazolyl blue) is a water soluble tetrazolium salt yielding a yellowish answer when ready in media or salt options missing phenol pink. Dissolved MTT is transformed to an insoluble purple formazan by cleavage of the tetrazolium ring by dehydrogenase enzymes. This water-insoluble formazan will be solubilized utilizing isopropanol or different solvents, and the dissolved materials is measured spectrophotometrically utilizing absorbance as a operate of focus of transformed dye.

XTT Cell Proliferation Assays

In distinction to MTT, the cleavage product of XTT is soluble in water; a solubilization step is subsequently not required. The tetrazolium salt XTT is cleaved to formazan by a fancy mobile mechanism. This bioreduction happens in viable cells solely, and is expounded to NAD(P)H manufacturing via glycolysis. The quantity of formazan dye fashioned straight correlates to the variety of metabolically energetic cells within the tradition.

WST-1 Cell Proliferation Assays

The steady tetrazolium salt WST-1 is cleaved to a soluble formazan by a fancy mobile mechanism that happens primarily on the cell floor. This bioreduction is basically depending on the glycolytic manufacturing of NAD(P)H in viable cells. The quantity of formazan dye fashioned straight correlates to the variety of metabolically energetic cells within the tradition.

 

BrdU Cell Proliferation Assay Kit
C0300-500 5000 Assays
EUR 4438
BrdU Cell Proliferation Assay Kit
K306-1000
EUR 974
BrdU Cell Proliferation Assay Kit
K306-200
EUR 392
CytoSelect BrdU Competitive ELISA Kit
CBA-5098 96 assays
EUR 543
CytoSelect MTT Cell Proliferation Assay
CBA-252 960 assays
EUR 409
Description: Cell Biolabs? CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation.  The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1).  An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNC552888-100 100uL
EUR 233
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF555 conjugate, Concentration: 0.1mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNC552888-500 500uL
EUR 545
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF555 conjugate, Concentration: 0.1mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNC612888-100 100uL
EUR 233
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF660R conjugate, Concentration: 0.1mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNC612888-500 500uL
EUR 545
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF660R conjugate, Concentration: 0.1mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNC402888-100 100uL
EUR 233
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF640R conjugate, Concentration: 0.1mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNC402888-500 500uL
EUR 545
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF640R conjugate, Concentration: 0.1mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNC472888-100 100uL
EUR 233
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF647 conjugate, Concentration: 0.1mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNC472888-500 500uL
EUR 545
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF647 conjugate, Concentration: 0.1mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNC052888-100 100uL
EUR 233
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF405M conjugate, Concentration: 0.1mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNC052888-500 500uL
EUR 545
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF405M conjugate, Concentration: 0.1mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNC042888-100 100uL
EUR 233
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF405S conjugate, Concentration: 0.1mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNC042888-500 500uL
EUR 545
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF405S conjugate, Concentration: 0.1mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNC432888-100 100uL
EUR 233
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF543 conjugate, Concentration: 0.1mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNC432888-500 500uL
EUR 545
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF543 conjugate, Concentration: 0.1mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNUB2888-100 100uL
EUR 264
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), Concentration: 0.2mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNUB2888-50 50uL
EUR 405
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), 1mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNUB2888-500 500uL
EUR 513
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), Concentration: 0.2mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNC682888-100 100uL
EUR 233
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF568 conjugate, Concentration: 0.1mg/mL
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody
BNC682888-500 500uL
EUR 545
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF568 conjugate, Concentration: 0.1mg/mL