Regulations for ballast water remedy specify limits on the concentrations of dwelling cells in discharge water. The very important stains fluorescein diacetate (FDA) and 5-chloromethylfluorescein diacetate (CMFDA) together have been beneficial to be used in verification of ballast water remedy know-how. We examined the effectiveness of FDA and CMFDA, singly and together, in discriminating between dwelling and heat-killed populations of 24 species of phytoplankton from seven divisions, verifying with quantitative progress assays that uniformly dwell and lifeless populations had been in contrast. The diagnostic sign, per-cell fluorescence depth, was measured by stream cytometry and alternate discriminatory thresholds had been outlined statistically from the frequency distributions of the lifeless or dwelling cells.
Species had been clustered by staining patterns: for 4 species, the staining of dwell versus lifeless cells was distinct, and live-dead classification was primarily error free. But overlap between the frequency distributions of dwelling and heat-killed cells within the different taxa led to unavoidable errors, nicely in extra of 20% in lots of. In Four very weakly staining taxa, the imply fluorescence depth within the heat-killed cells was larger than that of the dwelling cells, which is inconsistent with the assumptions of the strategy. Applying the standards of ≤5% false destructive plus ≤5% false constructive errors, and no important lack of cells resulting from staining, FDA and FDA+CMFDA gave acceptably correct outcomes for under 8-10 of 24 species (i.e., 33%-42%). CMFDA was the least efficient stain and its addition to FDA didn’t enhance the efficiency of FDA alone.

Classification of phytoplankton cells as live or dead using the vital stains fluorescein diacetate and 5-chloromethylfluorescein diacetate.
Design and synthesis of a luminescent cyclometalated iridium(III) advanced having N,N-diethylamino group that stains acidic intracellular organelles and induces celldemise by photoirradiation.
Cyclometalated iridium(III) complexes have obtained appreciable consideration and are essential candidates to be used as luminescent probes for mobile imaging due to their potential photophysical properties. We beforehand reported that fac-Ir(atpy)(3)4 (atpy = 2-(5′-amino-4′-tolyl)pyridine) containing three amino teams on the 5′-position of the atpy ligand exhibits a most purple emission (at round 600 nm) underneath impartial and fundamental situations and a inexperienced emission (at 531 nm) at acidic pH (pH 3-4). In this Article, we report on the design and synthesis of a brand new pH-sensitive cyclometalated Ir(III) advanced containing a 2-(5′-N,N-diethylamino-4′-tolyl)pyridine (deatpy) ligand, fac-Ir(deatpy)(3)5.
The advanced displays a substantial change in emission depth between impartial and barely acidic pH (pH 6.5-7.4). Luminescence microscopic research utilizing HeLa-S3 cells point out that 5 can be utilized to selectively stain lysosome, an acidic organelle in cells. Moreover, advanced 5 is able to producing singlet oxygen in a pH-dependent method and inducing the demise of HeLa-S3 cells upon photoirradiation at 377 or 470 nm.
Curious outcomes with palladium- and platinum-carrying polymers in mass cytometry bioassays and an surprising utility as a lifeless cell stain.
We describe the synthesis of metal-chelating polymers (MCPs) with 4 completely different pendant polyaminocarboxylate ligands (EDTA, DTPA, TTHA, DOTA) and an orthogonal end-group, both a fluorescein molecule or a bismaleimide linker for antibody attachment. Polymer characterization by a mix of (1)H NMR, UV/vis absorption measurements, and thermal gravimetric evaluation (TGA) indicated that every chain of the fluorescein-terminated polymers contained one dye molecule.
Fluorescein Isothiocyanate (FITC) Antibody |
abx020697-1mg |
Abbexa |
1 mg |
EUR 599 |
|
Fluorescein Isothiocyanate (FITC) Antibody |
abx020698-1mg |
Abbexa |
1 mg |
EUR 662 |
|
Fluorescein Isothiocyanate (FITC) Antibody |
abx020699-1mg |
Abbexa |
1 mg |
EUR 523 |
|
Fluorescein Isothiocyanate (FITC) Antibody |
abx022681-1mg |
Abbexa |
1 mg |
EUR 648 |
|
Fluorescein Isothiocyanate (FITC) Antibody |
20-abx178979 |
Abbexa |
-
EUR 439.00
-
EUR 133.00
-
EUR 1274.00
-
EUR 606.00
-
EUR 342.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Fluorescein Isothiocyanate (FITC) Antibody |
20-abx210188 |
Abbexa |
|
|
|
Gelatin, Fluorescein (FITC) Conjugate |
M1303-1 |
Biovision |
|
EUR 98 |
Gelatin, Fluorescein (FITC) Conjugate |
M1303-5 |
Biovision |
|
EUR 218 |
Collagen-Fluorescein (FITC) Conjugate |
M1304-1 |
Biovision |
|
EUR 98 |
Collagen-Fluorescein (FITC) Conjugate |
M1304-5 |
Biovision |
|
EUR 218 |
Fluorescein isothiocyanate, Isomer I [FITC] |
F742107 |
Bio Basic |
500mg |
EUR 168.32 |
|
Fluorescein isothiocyanate isomer I (FITC) |
abx082514-100mg |
Abbexa |
100 mg |
EUR 244 |
|
Fluorescein Isothiocyanate (FITC) Antibody (ALP) |
abx022679-1ml |
Abbexa |
1 ml |
EUR 718 |
|
Fluorescein Isothiocyanate (FITC) Antibody (HRP) |
abx022680-1ml |
Abbexa |
1 ml |
EUR 676 |
|
Fluorescein Isothiocyanate (FITC) Antibody (HRP) |
abx411353-1ml |
Abbexa |
1 ml |
EUR 690 |
|
Fluorescein Isothiocyanate (FITC) Antibody (HRP) |
abx411497-1ml |
Abbexa |
1 ml |
EUR 495 |
|
Fluorescein Isothiocyanate (FITC) Antibody (Biotin) |
abx411498-1mg |
Abbexa |
1 mg |
EUR 565 |
|
Fluorescein Isothiocyanate (FITC) Antibody (Biotin) |
abx412962-05mg |
Abbexa |
0.5 mg |
EUR 718 |
|
Fluorescein-5-isothiocyanate (FITC-I) |
20-abx182477 |
Abbexa |
-
EUR 398.00
-
EUR 2291.00
-
EUR 871.00
|
|
|
Fluorescein Isothiocyanate (FITC) Protein (OVA) |
20-abx651911 |
Abbexa |
-
EUR 578.00
-
EUR 258.00
-
EUR 1720.00
-
EUR 690.00
-
EUR 425.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
OVA Conjugated Fluorescein Isothiocyanate (FITC) |
4-CPO843Ge21 |
Cloud-Clone |
-
EUR 413.60
-
EUR 214.00
-
EUR 1276.00
-
EUR 492.00
-
EUR 884.00
-
EUR 340.00
-
EUR 3040.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Pan-species Fluorescein Isothiocyanate expressed in: E.coli |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNCA1277-250 |
Biotium |
250uL |
EUR 383 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),APC conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC811277-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF680R conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC811277-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF680R conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNCAP1277-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNCAP1277-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNCB1277-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),Biotin conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNCB1277-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),Biotin conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC471277-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF647 conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC471277-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF647 conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC551277-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF555 conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC551277-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF555 conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC431277-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF543 conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC431277-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF543 conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC881277-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF488A conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC881277-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF488A conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC941277-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF594 conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC941277-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF594 conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNCH1277-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNCH1277-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNCP1277-250 |
Biotium |
250uL |
EUR 383 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),PerCP conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC041277-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF405S conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC041277-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF405S conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNCR1277-250 |
Biotium |
250uL |
EUR 383 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),RPE conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNUB1277-100 |
Biotium |
100uL |
EUR 209 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1), Concentration: 0.2mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNUB1277-500 |
Biotium |
500uL |
EUR 458 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1), Concentration: 0.2mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC681277-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF568 conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC681277-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF568 conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC701277-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF770 conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC701277-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF770 conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC051277-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF405M conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC051277-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF405M conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC611277-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF660R conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC611277-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF660R conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC401277-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF640R conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC401277-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF640R conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC801277-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF680 conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNC801277-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF680 conjugate, Concentration: 0.1mg/mL |
FITC (Fluorescein Isothiocyanate) (F4/1) Antibody |
BNUM1277-50 |
Biotium |
50uL |
EUR 395 |
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1), 1mg/mL |
Fluorescein Isothiocyanate (FITC) Monoclonal Antibody (Pan-species), FITC |
4-MAO843Ge21-FITC |
Cloud-Clone |
-
EUR 297.00
-
EUR 2654.00
-
EUR 753.00
-
EUR 373.00
-
EUR 196.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (FITC). This antibody is labeled with FITC. |
Fluorescein Isothiocyanate (FITC) Monoclonal Antibody (Pan-species) |
4-MAO843Ge21 |
Cloud-Clone |
-
EUR 247.00
-
EUR 2523.00
-
EUR 628.00
-
EUR 311.00
-
EUR 214.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (FITC) |
Fluorescein diacetate |
FB0202 |
Bio Basic |
5g |
EUR 158.75 |
|
Fluorescein diacetate |
abx082098-1g |
Abbexa |
1 g |
EUR 203 |
|
Fluorescein-phalloidin |
00030 |
Biotium |
300U |
EUR 345 |
Description: Minimum order quantity: 1 unit of 300U |
fluorescein biotin |
80019 |
Biotium |
5MG |
EUR 170 |
Description: Minimum order quantity: 1 unit of 5MG |
Fluorescein hexylamine |
91058 |
Biotium |
25MG |
EUR 217 |
Description: Minimum order quantity: 1 unit of 25MG |
Fluorescein tyramide |
96018 |
Biotium |
0.5mg |
EUR 339 |
Description: Minimum order quantity: 1 unit of 0.5mg |
Fluorescein Isothiocyanate (FITC) Monoclonal Antibody (Pan-species), APC |
4-MAO843Ge21-APC |
Cloud-Clone |
-
EUR 346.00
-
EUR 3293.00
-
EUR 917.00
-
EUR 441.00
-
EUR 220.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (FITC). This antibody is labeled with APC. |
Fluorescein Isothiocyanate (FITC) Monoclonal Antibody (Pan-species), Biotinylated |
4-MAO843Ge21-Biotin |
Cloud-Clone |
-
EUR 312.00
-
EUR 2473.00
-
EUR 730.00
-
EUR 382.00
-
EUR 219.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (FITC). This antibody is labeled with Biotin. |
Fluorescein Isothiocyanate (FITC) Monoclonal Antibody (Pan-species), HRP |
4-MAO843Ge21-HRP |
Cloud-Clone |
-
EUR 316.00
-
EUR 2870.00
-
EUR 811.00
-
EUR 399.00
-
EUR 207.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (FITC). This antibody is labeled with HRP. |
Fluorescein, disodium salt |
FB0203 |
Bio Basic |
100g |
EUR 63.92 |
|
Fluorescein, free acid |
FB0463 |
Bio Basic |
100g |
EUR 63.92 |
|
Ret-Fluorescein Labeled |
FC15016 |
Neuromics |
100 Tests |
EUR 448 |
Endoglin/CD105 Fluorescein |
FC15022 |
Neuromics |
100 Tests |
EUR 448 |
Fluorescein-Alpha-bungarotoxin |
00011 |
Biotium |
500uG |
EUR 367 |
Description: Minimum order quantity: 1 unit of 500uG |
Fluorescein-DHPE: (5mg) |
60024 |
Biotium |
5MG |
EUR 226 |
Description: Minimum order quantity: 1 unit of 5MG |
biotin-4-fluorescein |
90062 |
Biotium |
10MG |
EUR 188 |
Description: Minimum order quantity: 1 unit of 10MG |
fluorescein-5-maleimide |
91028 |
Biotium |
25MG |
EUR 266 |
Description: Minimum order quantity: 1 unit of 25MG |
Fluorescein-12-dUTP |
B8207-.025 |
ApexBio |
25 ?l (1mM) |
EUR 282 |
Description: Fluorescein-12-dUTP could be used in directly enzymatic labeling of DNA/cDNA, such as PCR, random primed DNA labeling reaction, primer extension, Nick Translation and others. It can be incorporated as a substitute for its natural counterpart dTTP. |
Fluorescein-12-dUTP |
B8207-.125 |
ApexBio |
5x25 ?l (1mM) |
EUR 1024 |
Description: Fluorescein-12-dUTP could be used in directly enzymatic labeling of DNA/cDNA, such as PCR, random primed DNA labeling reaction, primer extension, Nick Translation and others. It can be incorporated as a substitute for its natural counterpart dTTP. |
These polymer samples had been loaded with three several types of lanthanide ions in addition to palladium and platinum ions. The numbers of metallic atoms per chain had been decided by a mix of UV/vis and standard ICP-MS measurements. The experiments with lanthanide ions demonstrated {that a} web anionic cost on the polymer is essential for water solubility. These experiments additionally confirmed that no less than one kind of lanthanide ion (La(3+)) is able to forming a bimetallic advanced with pendant DTPA teams.