Classification of phytoplankton cells as live or dead using the vital stains fluorescein diacetate and 5-chloromethylfluorescein diacetate.

522  Nathan Holland  
(last edited )

Regulations for ballast water remedy specify limits on the concentrations of dwelling cells in discharge water. The very important stains fluorescein diacetate (FDA) and 5-chloromethylfluorescein diacetate (CMFDA) together have been beneficial to be used in verification of ballast water remedy know-how. We examined the effectiveness of FDA and CMFDA, singly and together, in discriminating between dwelling and heat-killed populations of 24 species of phytoplankton from seven divisions, verifying with quantitative progress assays that uniformly dwell and lifeless populations had been in contrast. The diagnostic sign, per-cell fluorescence depth, was measured by stream cytometry and alternate discriminatory thresholds had been outlined statistically from the frequency distributions of the lifeless or dwelling cells.

Species had been clustered by staining patterns: for 4 species, the staining of dwell versus lifeless cells was distinct, and live-dead classification was primarily error free. But overlap between the frequency distributions of dwelling and heat-killed cells within the different taxa led to unavoidable errors, nicely in extra of 20% in lots of. In Four very weakly staining taxa, the imply fluorescence depth within the heat-killed cells was larger than that of the dwelling cells, which is inconsistent with the assumptions of the strategy. Applying the standards of ≤5% false destructive plus ≤5% false constructive errors, and no important lack of cells resulting from staining, FDA and FDA+CMFDA gave acceptably correct outcomes for under 8-10 of 24 species (i.e., 33%-42%). CMFDA was the least efficient stain and its addition to FDA didn’t enhance the efficiency of FDA alone.

Classification of phytoplankton cells as live or dead using the vital stains fluorescein diacetate and 5-chloromethylfluorescein diacetate.

Classification of phytoplankton cells as live or dead using the vital stains fluorescein diacetate and 5-chloromethylfluorescein diacetate.

Design and synthesis of a luminescent cyclometalated iridium(III) advanced having N,N-diethylamino group that stains acidic intracellular organelles and induces celldemise by photoirradiation.

Cyclometalated iridium(III) complexes have obtained appreciable consideration and are essential candidates to be used as luminescent probes for mobile imaging due to their potential photophysical properties. We beforehand reported that fac-Ir(atpy)(3)4 (atpy = 2-(5′-amino-4′-tolyl)pyridine) containing three amino teams on the 5′-position of the atpy ligand exhibits a most purple emission (at round 600 nm) underneath impartial and fundamental situations and a inexperienced emission (at 531 nm) at acidic pH (pH 3-4). In this Article, we report on the design and synthesis of a brand new pH-sensitive cyclometalated Ir(III) advanced containing a 2-(5′-N,N-diethylamino-4′-tolyl)pyridine (deatpy) ligand, fac-Ir(deatpy)(3)5.
The advanced displays a substantial change in emission depth between impartial and barely acidic pH (pH 6.5-7.4). Luminescence microscopic research utilizing HeLa-S3 cells point out that 5 can be utilized to selectively stain lysosome, an acidic organelle in cells. Moreover, advanced 5 is able to producing singlet oxygen in a pH-dependent method and inducing the demise of HeLa-S3 cells upon photoirradiation at 377 or 470 nm.

Curious outcomes with palladium- and platinum-carrying polymers in mass cytometry bioassays and an surprising utility as a lifeless cell stain.

We describe the synthesis of metal-chelating polymers (MCPs) with 4 completely different pendant polyaminocarboxylate ligands (EDTA, DTPA, TTHA, DOTA) and an orthogonal end-group, both a fluorescein molecule or a bismaleimide linker for antibody attachment. Polymer characterization by a mix of (1)H NMR, UV/vis absorption measurements, and thermal gravimetric evaluation (TGA) indicated that every chain of the fluorescein-terminated polymers contained one dye molecule.

Fluorescein Isothiocyanate (FITC) Antibody
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Gelatin, Fluorescein (FITC) Conjugate
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OVA Conjugated Fluorescein Isothiocyanate (FITC)
4-CPO843Ge21
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  • Uniprot ID: Inquire
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 389.4g/mol
  • Isoelectric Point: Inquire
Description: Recombinant Pan-species Fluorescein Isothiocyanate expressed in: E.coli

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Fluorescein Isothiocyanate (FITC) Protein (OVA)
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Fluorescein isothiocyanate, Isomer I [FITC]
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Fluorescein
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FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNUM1277-50 50uL
EUR 395.00
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FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNUB1277-100 100uL
EUR 209.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1), Concentration: 0.2mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNUB1277-500 500uL
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Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1), Concentration: 0.2mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC551277-100 100uL
EUR 199.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF555 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC551277-500 500uL
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FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC611277-100 100uL
EUR 199.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF660R conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC611277-500 500uL
EUR 544.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF660R conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC471277-100 100uL
EUR 199.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF647 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC471277-500 500uL
EUR 544.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF647 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC051277-100 100uL
EUR 199.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF405M conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC051277-500 500uL
EUR 544.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF405M conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC401277-100 100uL
EUR 199.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF640R conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC401277-500 500uL
EUR 544.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF640R conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC431277-100 100uL
EUR 199.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF543 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC431277-500 500uL
EUR 544.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF543 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC041277-100 100uL
EUR 199.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF405S conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC041277-500 500uL
EUR 544.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF405S conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC701277-100 100uL
EUR 199.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF770 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC701277-500 500uL
EUR 544.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF770 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC801277-100 100uL
EUR 199.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF680 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC801277-500 500uL
EUR 544.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF680 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNCP1277-250 250uL
EUR 383.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),PerCP conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNCR1277-250 250uL
EUR 383.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),RPE conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNCA1277-250 250uL
EUR 383.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),APC conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNCB1277-100 100uL
EUR 199.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),Biotin conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNCB1277-500 500uL
EUR 544.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),Biotin conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNCH1277-100 100uL
EUR 199.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNCH1277-500 500uL
EUR 544.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC881277-100 100uL
EUR 199.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF488A conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC881277-500 500uL
EUR 544.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF488A conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC941277-100 100uL
EUR 199.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF594 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC941277-500 500uL
EUR 544.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF594 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC681277-100 100uL
EUR 199.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF568 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC681277-500 500uL
EUR 544.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF568 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNCAP1277-100 100uL
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FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNCAP1277-500 500uL
EUR 544.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC811277-100 100uL
EUR 199.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF680R conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody
BNC811277-500 500uL
EUR 544.00
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF680R conjugate, Concentration: 0.1mg/mL

Fluorescein Isothiocyanate (FITC) Monoclonal Antibody (Pan-species), FITC
4-MAO843Ge21-FITC
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  • EUR 196.00
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  • 4
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (FITC). This antibody is labeled with FITC.

Fluorescein Isothiocyanate (FITC) Monoclonal Antibody (Pan-species)
4-MAO843Ge21
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  • EUR 214.00
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  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (FITC)

Fluorescein DHPE
23304 5 mg
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  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

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FB0202 5g
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Fluorescein Isothiocyanate (FITC) Monoclonal Antibody (Pan-species), APC
4-MAO843Ge21-APC
  • EUR 346.00
  • EUR 3293.00
  • EUR 917.00
  • EUR 441.00
  • EUR 220.00
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  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (FITC). This antibody is labeled with APC.

Fluorescein Isothiocyanate (FITC) Monoclonal Antibody (Pan-species), Biotinylated
4-MAO843Ge21-Biotin
  • EUR 312.00
  • EUR 2473.00
  • EUR 730.00
  • EUR 382.00
  • EUR 219.00
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  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (FITC). This antibody is labeled with Biotin.

Fluorescein Isothiocyanate (FITC) Monoclonal Antibody (Pan-species), HRP
4-MAO843Ge21-HRP
  • EUR 316.00
  • EUR 2870.00
  • EUR 811.00
  • EUR 399.00
  • EUR 207.00
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  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (FITC). This antibody is labeled with HRP.

Phalloidin-Fluorescein Conjugate
23101 300 Tests
EUR 176.00
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

Fluorescein-Alpha-bungarotoxin
00011 500uG
EUR 367.00
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Fluorescein-DHPE: (5mg)
60024 5MG
EUR 226.00
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HCV Nucleocapsid [Fluorescein]
DAG1389 100 µg
EUR 810.00

biotin-4-fluorescein
90062 10MG
EUR 188.00
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fluorescein-5-maleimide
91028 25MG
EUR 266.00
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Fluorescein-12-dUTP
B8207-.025 25 ?l (1mM)
EUR 282.00
Description: Fluorescein-12-dUTP could be used in directly enzymatic labeling of DNA/cDNA, such as PCR, random primed DNA labeling reaction, primer extension, Nick Translation and others. It can be incorporated as a substitute for its natural counterpart dTTP.

Fluorescein-12-dUTP
B8207-.125 5x25 ?l (1mM)
EUR 1024.00
Description: Fluorescein-12-dUTP could be used in directly enzymatic labeling of DNA/cDNA, such as PCR, random primed DNA labeling reaction, primer extension, Nick Translation and others. It can be incorporated as a substitute for its natural counterpart dTTP.

Fluorescein-5-maleimide
HY-D0098 25mg
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Fluorescein (C.I. 45350)
GT3509-100G 100 g
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Fluorescein (C.I. 45350)
GT3509-500G 500 g
EUR 94.00

Ret-Fluorescein Labeled
FC15016 100 Tests
EUR 448.00

Endoglin/CD105 Fluorescein
FC15022 100 Tests
EUR 448.00

5-(Pentafluorobenzoylamino)fluorescein
M-2375.0005 5.0mg
EUR 225.00
Description: Sum Formula: C27H12F5NO6; CAS# [209540-56-7]

5-Carboxy-fluorescein
Q-2660.0100 100.0mg
EUR 126.00
Description: Sum Formula: C21H12O7; CAS# [76823-03-5]

5-Carboxy-fluorescein
Q-2660.0500 500.0mg
EUR 466.00
Description: Sum Formula: C21H12O7; CAS# [76823-03-5]

6-Carboxy-fluorescein
Q-2665.0100 100.0mg
EUR 126.00
Description: Sum Formula: C21H12O7; CAS# [3301-79-9]

6-Carboxy-fluorescein
Q-2665.0500 500.0mg
EUR 466.00
Description: Sum Formula: C21H12O7; CAS# [3301-79-9]

Fluorescein, disodium salt
FB0203 100g
EUR 63.92
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Fluorescein, free acid
FB0463 100g
EUR 63.92
  • Product category: Biochemicals/Indicators/Stains/Other

CF405M monoclonal mouse anti-fluorescein (FITC) IgG, 2mg/mL
20214 0.25mL
EUR 277.00
Description: Minimum order quantity: 1 unit of 0.25mL

CF405M monoclonal mouse anti-fluorescein (FITC) IgG, 2mg/mL
20214-1 50uL
EUR 161.00
Description: Minimum order quantity: 1 unit of 50uL

CF488A monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL
20210 0.25mL
EUR 277.00
Description: Minimum order quantity: 1 unit of 0.25mL

CF488A monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL
20210-1 50uL
EUR 161.00
Description: Minimum order quantity: 1 unit of 50uL

CF594 monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL
20211 0.25mL
EUR 277.00
Description: Minimum order quantity: 1 unit of 0.25mL

CF633 monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL
20212 0.25mL
EUR 277.00
Description: Minimum order quantity: 1 unit of 0.25mL

CF640R monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL
20213 0.25mL
EUR 277.00
Description: Minimum order quantity: 1 unit of 0.25mL

CF660R monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL
20399-0.25mL 0.25mL
EUR 277.00
Description: Minimum order quantity: 1 unit of 0.25mL

CF660R monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL
20399-50uL 50uL
EUR 161.00
Description: Minimum order quantity: 1 unit of 50uL

Fluorescein cadaverine (5-((5-aminopentyl) thioureidyl)fluorescein, trifluoroacetate salt): (25mg)
92000 25MG
EUR 230.00
Description: Minimum order quantity: 1 unit of 25MG

Monoclonal Anti-Rat IgG1-FITC Conjugate
50126-FITC 0.5 ml
EUR 286.00

Concanavalin A Lectin (ConA)-FITC conjugate
CONA15-FITC 0.5 ml
EUR 286.00

Aleuria Aurantia Lectin (Aal)-FITC conjugate
AAL15-FITC 0.5 ml
EUR 225.00

Lens culinaris Lectin (LCA)-FITC conjugate
LCA15-FITC 0.5 ml
EUR 286.00

Rabbit Anti-Salmonella IgG-FITC conjugate
SLM11-FITC 0.5 ml
EUR 347.00

Sambucus nigra Lectin (SNA)-FITC conjugate
SNA15-FITC 0.5 ml
EUR 286.00

CLF [Cholyl-Lys-Fluorescein]
36701 1 mg
EUR 306.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

AMP-Fluorescein conjugate calibrator
13610 100 nmol
EUR 306.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

GMP-Fluorescein conjugate calibrator
13611 100 nmol
EUR 480.00
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Fluorescein-alpha-bungarotoxin: (10x50ug)
00013 10ST
EUR 417.00
Description: Minimum order quantity: 1 unit of 10ST

Fluorescein Methotrexate, triammonium salt:
00023 1MG
EUR 281.00
Description: Minimum order quantity: 1 unit of 1MG

SensiFAST SYBR & Fluorescein Kit
BIO-96002/S Sample Ask for price

SensiFAST SYBR & Fluorescein Kit
BIO-96005 500 rxns Ask for price

SensiFAST SYBR & Fluorescein Kit
BIO-96020 2000 rxns Ask for price

Fluorescein 5(6)-isothiocyanate
GT8666-1G 1 g
EUR 309.00

Fluorescein Reference Dye (100X)
F553 1 ml (10µM)
EUR 61.00

Fluorescein Reference Dye (100X)
F553L 5x1 ml (50µM)
EUR 172.00

FGF R3-Fluorescein Labeled
FC15006 100 Tests
EUR 448.00

SensiMix SYBR & Fluorescein Kit
QT615-05 500 rxn Ask for price

SensiMix SYBR & Fluorescein Kit
QT615-20 2000 rxn Ask for price

Goat Anti-Mouse albumin IgG-FITC Conjugate
ALB11-FITC 100 ug
EUR 347.00

Goat Anti-Human albumin IgG-FITC Conjugate
ALBH13-FITC 100 ug
EUR 347.00

Recombinant (E.Coli) purified Protein G-FITC Conjugate
PRTG15-FITC 1 ml
EUR 202.00
These polymer samples had been loaded with three several types of lanthanide ions in addition to palladium and platinum ions. The numbers of metallic atoms per chain had been decided by a mix of UV/vis and standard ICP-MS measurements. The experiments with lanthanide ions demonstrated {that a} web anionic cost on the polymer is essential for water solubility. These experiments additionally confirmed that no less than one kind of lanthanide ion (La(3+)) is able to forming a bimetallic advanced with pendant DTPA teams.