Regulations for ballast water remedy specify limits on the concentrations of dwelling cells in discharge water. The very important stains fluorescein diacetate (FDA) and 5-chloromethylfluorescein diacetate (CMFDA) together have been beneficial to be used in verification of ballast water remedy know-how. We examined the effectiveness of FDA and CMFDA, singly and together, in discriminating between dwelling and heat-killed populations of 24 species of phytoplankton from seven divisions, verifying with quantitative progress assays that uniformly dwell and lifeless populations had been in contrast. The diagnostic sign, per-cell fluorescence depth, was measured by stream cytometry and alternate discriminatory thresholds had been outlined statistically from the frequency distributions of the lifeless or dwelling cells.

Species had been clustered by staining patterns: for 4 species, the staining of dwell versus lifeless cells was distinct, and live-dead classification was primarily error free. But overlap between the frequency distributions of dwelling and heat-killed cells within the different taxa led to unavoidable errors, nicely in extra of 20% in lots of. In Four very weakly staining taxa, the imply fluorescence depth within the heat-killed cells was larger than that of the dwelling cells, which is inconsistent with the assumptions of the strategy. Applying the standards of ≤5% false destructive plus ≤5% false constructive errors, and no important lack of cells resulting from staining, FDA and FDA+CMFDA gave acceptably correct outcomes for under 8-10 of 24 species (i.e., 33%-42%). CMFDA was the least efficient stain and its addition to FDA didn’t enhance the efficiency of FDA alone.

 Classification of phytoplankton cells as live or dead using the vital stains fluorescein diacetate and 5-chloromethylfluorescein diacetate.


Classification of phytoplankton cells as live or dead using the vital stains fluorescein diacetate and 5-chloromethylfluorescein diacetate.

Design and synthesis of a luminescent cyclometalated iridium(III) advanced having N,N-diethylamino group that stains acidic intracellular organelles and induces celldemise by photoirradiation.

Cyclometalated iridium(III) complexes have obtained appreciable consideration and are essential candidates to be used as luminescent probes for mobile imaging due to their potential photophysical properties. We beforehand reported that fac-Ir(atpy)(3)4 (atpy = 2-(5′-amino-4′-tolyl)pyridine) containing three amino teams on the 5′-position of the atpy ligand exhibits a most purple emission (at round 600 nm) underneath impartial and fundamental situations and a inexperienced emission (at 531 nm) at acidic pH (pH 3-4). In this Article, we report on the design and synthesis of a brand new pH-sensitive cyclometalated Ir(III) advanced containing a 2-(5′-N,N-diethylamino-4′-tolyl)pyridine (deatpy) ligand, fac-Ir(deatpy)(3)5.
The advanced displays a substantial change in emission depth between impartial and barely acidic pH (pH 6.5-7.4). Luminescence microscopic research utilizing HeLa-S3 cells point out that 5 can be utilized to selectively stain lysosome, an acidic organelle in cells. Moreover, advanced 5 is able to producing singlet oxygen in a pH-dependent method and inducing the demise of HeLa-S3 cells upon photoirradiation at 377 or 470 nm.

Curious outcomes with palladium- and platinum-carrying polymers in mass cytometry bioassays and an surprising utility as a lifeless cell stain.

We describe the synthesis of metal-chelating polymers (MCPs) with 4 completely different pendant polyaminocarboxylate ligands (EDTA, DTPA, TTHA, DOTA) and an orthogonal end-group, both a fluorescein molecule or a bismaleimide linker for antibody attachment. Polymer characterization by a mix of (1)H NMR, UV/vis absorption measurements, and thermal gravimetric evaluation (TGA) indicated that every chain of the fluorescein-terminated polymers contained one dye molecule.

Fluorescein Isothiocyanate (FITC) Antibody

abx020697-1mg 1 mg
EUR 599

Fluorescein Isothiocyanate (FITC) Antibody

abx020698-1mg 1 mg
EUR 662

Fluorescein Isothiocyanate (FITC) Antibody

abx020699-1mg 1 mg
EUR 523

Fluorescein Isothiocyanate (FITC) Antibody

abx022681-1mg 1 mg
EUR 648

Fluorescein Isothiocyanate (FITC) Antibody

20-abx178979
  • EUR 439.00
  • EUR 133.00
  • EUR 1274.00
  • EUR 606.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Fluorescein Isothiocyanate (FITC) Antibody

20-abx210188
  • EUR 439.00
  • EUR 328.00
  • 100 ug
  • 50 ug

Streptavidin-Fluorescein (FITC) conjugate

20367 0.5 mg
EUR 202

Gelatin, Fluorescein (FITC) Conjugate

M1303-1
EUR 98

Gelatin, Fluorescein (FITC) Conjugate

M1303-5
EUR 218

Collagen-Fluorescein (FITC) Conjugate

M1304-1
EUR 98

Collagen-Fluorescein (FITC) Conjugate

M1304-5
EUR 218

Fluorescein isothiocyanate, Isomer I [FITC]

F742107 500mg
EUR 168.32

Fluorescein isothiocyanate isomer I (FITC)

abx082514-100mg 100 mg
EUR 244

Fluorescein Isothiocyanate (FITC) Antibody (ALP)

abx022679-1ml 1 ml
EUR 718

Fluorescein Isothiocyanate (FITC) Antibody (HRP)

abx022680-1ml 1 ml
EUR 676

Fluorescein Isothiocyanate (FITC) Antibody (HRP)

abx411353-1ml 1 ml
EUR 690

Fluorescein Isothiocyanate (FITC) Antibody (HRP)

abx411497-1ml 1 ml
EUR 495

Fluorescein Isothiocyanate (FITC) Antibody (Biotin)

abx411498-1mg 1 mg
EUR 565

Fluorescein Isothiocyanate (FITC) Antibody (Biotin)

abx412962-05mg 0.5 mg
EUR 718

Fluorescein-5-isothiocyanate (FITC-I)

20-abx182477
  • EUR 398.00
  • EUR 2291.00
  • EUR 871.00
  • 1 g
  • 25 g
  • 5 g

Fluorescein Isothiocyanate (FITC) Protein (OVA)

20-abx651911
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

OVA Conjugated Fluorescein Isothiocyanate (FITC)

4-CPO843Ge21
  • EUR 413.60
  • EUR 214.00
  • EUR 1276.00
  • EUR 492.00
  • EUR 884.00
  • EUR 340.00
  • EUR 3040.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pan-species Fluorescein Isothiocyanate expressed in: E.coli

Fluorescein

HY-D0251 1g
EUR 139

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNCA1277-250 250uL
EUR 383
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),APC conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC811277-100 100uL
EUR 199
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF680R conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC811277-500 500uL
EUR 544
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF680R conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNCAP1277-100 100uL
EUR 199
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNCAP1277-500 500uL
EUR 544
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNCB1277-100 100uL
EUR 199
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),Biotin conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNCB1277-500 500uL
EUR 544
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),Biotin conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC471277-100 100uL
EUR 199
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF647 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC471277-500 500uL
EUR 544
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF647 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC551277-100 100uL
EUR 199
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF555 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC551277-500 500uL
EUR 544
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF555 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC431277-100 100uL
EUR 199
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF543 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC431277-500 500uL
EUR 544
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF543 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC881277-100 100uL
EUR 199
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF488A conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC881277-500 500uL
EUR 544
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF488A conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC941277-100 100uL
EUR 199
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF594 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC941277-500 500uL
EUR 544
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF594 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNCH1277-100 100uL
EUR 199
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNCH1277-500 500uL
EUR 544
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNCP1277-250 250uL
EUR 383
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),PerCP conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC041277-100 100uL
EUR 199
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF405S conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC041277-500 500uL
EUR 544
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF405S conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNCR1277-250 250uL
EUR 383
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),RPE conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNUB1277-100 100uL
EUR 209
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1), Concentration: 0.2mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNUB1277-500 500uL
EUR 458
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1), Concentration: 0.2mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC681277-100 100uL
EUR 199
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF568 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC681277-500 500uL
EUR 544
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF568 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC701277-100 100uL
EUR 199
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF770 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC701277-500 500uL
EUR 544
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF770 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC051277-100 100uL
EUR 199
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF405M conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC051277-500 500uL
EUR 544
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF405M conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC611277-100 100uL
EUR 199
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF660R conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC611277-500 500uL
EUR 544
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF660R conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC401277-100 100uL
EUR 199
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF640R conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC401277-500 500uL
EUR 544
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF640R conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC801277-100 100uL
EUR 199
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF680 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNC801277-500 500uL
EUR 544
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1),CF680 conjugate, Concentration: 0.1mg/mL

FITC (Fluorescein Isothiocyanate) (F4/1) Antibody

BNUM1277-50 50uL
EUR 395
Description: Primary antibody against FITC (Fluorescein Isothiocyanate) (F4/1), 1mg/mL

Fluorescein Isothiocyanate (FITC) Monoclonal Antibody (Pan-species), FITC

4-MAO843Ge21-FITC
  • EUR 297.00
  • EUR 2654.00
  • EUR 753.00
  • EUR 373.00
  • EUR 196.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (FITC). This antibody is labeled with FITC.

Fluorescein Isothiocyanate (FITC) Monoclonal Antibody (Pan-species)

4-MAO843Ge21
  • EUR 247.00
  • EUR 2523.00
  • EUR 628.00
  • EUR 311.00
  • EUR 214.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (FITC)

Fluorescein diacetate

FB0202 5g
EUR 158.75

Fluorescein Diacetate

HY-D0719 5g
EUR 119

Fluorescein Biotin

HY-D1030 1mg
EUR 201

Fluorescein Phalloidin

HY-K0902 300T
EUR 843

Fluorescein diacetate

abx082098-1g 1 g
EUR 203

Fluorescein-phalloidin

00030 300U
EUR 345
Description: Minimum order quantity: 1 unit of 300U

fluorescein biotin

80019 5MG
EUR 170
Description: Minimum order quantity: 1 unit of 5MG

Fluorescein hexylamine

91058 25MG
EUR 217
Description: Minimum order quantity: 1 unit of 25MG

Fluorescein tyramide

96018 0.5mg
EUR 339
Description: Minimum order quantity: 1 unit of 0.5mg

Fluorescein diacetate

GT5229-100G 100 g
EUR 524

Fluorescein diacetate

GT5229-10G 10 g
EUR 99

Fluorescein diacetate

GT5229-25G 25 g
EUR 181

Fluorescein diacetate

GT5229-5G 5 g
EUR 70

Fluorescein DHPE

23304 5 mg
EUR 132

Fluorescein Isothiocyanate (FITC) Monoclonal Antibody (Pan-species), APC

4-MAO843Ge21-APC
  • EUR 346.00
  • EUR 3293.00
  • EUR 917.00
  • EUR 441.00
  • EUR 220.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (FITC). This antibody is labeled with APC.

Fluorescein Isothiocyanate (FITC) Monoclonal Antibody (Pan-species), Biotinylated

4-MAO843Ge21-Biotin
  • EUR 312.00
  • EUR 2473.00
  • EUR 730.00
  • EUR 382.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (FITC). This antibody is labeled with Biotin.

Fluorescein Isothiocyanate (FITC) Monoclonal Antibody (Pan-species), HRP

4-MAO843Ge21-HRP
  • EUR 316.00
  • EUR 2870.00
  • EUR 811.00
  • EUR 399.00
  • EUR 207.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (FITC). This antibody is labeled with HRP.

Fluorescein, disodium salt

FB0203 100g
EUR 63.92

Fluorescein, free acid

FB0463 100g
EUR 63.92

HCV Nucleocapsid [Fluorescein]

DAG1389 100 µg
EUR 810

Fluorescein-5-maleimide

HY-D0098 25mg
EUR 187

Ret-Fluorescein Labeled

FC15016 100 Tests
EUR 448

Endoglin/CD105 Fluorescein

FC15022 100 Tests
EUR 448

Fluorescein-Alpha-bungarotoxin

00011 500uG
EUR 367
Description: Minimum order quantity: 1 unit of 500uG

Fluorescein-DHPE: (5mg)

60024 5MG
EUR 226
Description: Minimum order quantity: 1 unit of 5MG

biotin-4-fluorescein

90062 10MG
EUR 188
Description: Minimum order quantity: 1 unit of 10MG

fluorescein-5-maleimide

91028 25MG
EUR 266
Description: Minimum order quantity: 1 unit of 25MG

Fluorescein-12-dUTP

B8207-.025 25 ?l (1mM)
EUR 282
Description: Fluorescein-12-dUTP could be used in directly enzymatic labeling of DNA/cDNA, such as PCR, random primed DNA labeling reaction, primer extension, Nick Translation and others. It can be incorporated as a substitute for its natural counterpart dTTP.

Fluorescein-12-dUTP

B8207-.125 5x25 ?l (1mM)
EUR 1024
Description: Fluorescein-12-dUTP could be used in directly enzymatic labeling of DNA/cDNA, such as PCR, random primed DNA labeling reaction, primer extension, Nick Translation and others. It can be incorporated as a substitute for its natural counterpart dTTP.

Fluorescein (C.I. 45350)

GT3509-100G 100 g
EUR 54

Fluorescein (C.I. 45350)

GT3509-500G 500 g
EUR 94
These polymer samples had been loaded with three several types of lanthanide ions in addition to palladium and platinum ions. The numbers of metallic atoms per chain had been decided by a mix of UV/vis and standard ICP-MS measurements. The experiments with lanthanide ions demonstrated {that a} web anionic cost on the polymer is essential for water solubility. These experiments additionally confirmed that no less than one kind of lanthanide ion (La(3+)) is able to forming a bimetallic advanced with pendant DTPA teams.