General description

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue mild inflicting molecules to turn into excited and emit a visual yellow-green mild. This emission ceases upon removing of the sunshine inflicting the excitation. Fluorochrome labeling gives fast, correct localization of antigen-antibody interplay when one of many reactants is a part of a cell, tissue or different organic construction. FITC is a generally used marker for antibodies in immunofluorescent strategies as a result of the conjugation of FITC to proteins is comparatively simple and customarily doesn’t destroy the organic exercise of the labeled protein. FITC is broadly used as a hapten to label totally different proteins. Antibodies to FITC are used to establish FITC labeled proteins and as fashions to review the mechanism of antibody response to a nicely outlined hapten.

Specificity

The antibody reacts with each free and conjugated fluorescein isothiocyanate (FITC).
Mouse monoclonal clone FL-D6 anti-FITC antibody will react with both free or conjugated FITC. The antibody doesn’t react with sure or free TRITC (tetramethylrhodamine isothiocyanate).

Immunogen

FITC-BSA conjugate

Application

Mouse monoclonal clone FL-D6 anti-FITC antibody could also be used for the detection of FITC and as a common indicator reagent for bridging FITC with different immunochemical reagents. It could also be utilized in ELISA, aggressive ELISA and immunofluorescent strategies. A FITC Anti-FITC system has been used within the amplification of sign in immunofluorescent detection and as a method of separating sure from free tracer by affinity chromatography. The antibody may also be used to isolate cells which have an FITC-labeled ligand on their floor.
fd-rapid-golgistain-kit

fd-rapid-golgistain-kit

Disclaimer

Unless in any other case acknowledged in our catalog or different firm documentation accompanying the product(s), our merchandise are supposed for analysis use solely and will not be for use for some other objective, which incorporates however shouldn’t be restricted to, unauthorized industrial makes use of, in vitro diagnostic makes use of, ex vivo or in vivo therapeutic makes use of or any kind of consumption or utility to people or animals.

Features

Mouse Monoclonal Antibody

Elabscience’s expert technicians put together mouse monoclonal antibodies with first-class experimental tools and optimization operations, which may promise the final word consistency and efficiency.

Western Blot Detection Kits

Elabscience presents Western Blot Detection Kits which offered for classical Western Blot experiments, together with all reagents wanted from protein extraction to outcome detection, which have benefits of easy operation, excessive detection sensitivity, low background and robust system stability. And Elabscience® gives detailed operation description {and professional} technical help. Additionally, it presents you comfort and assure to work out preferrred Western Blot experiment outcomes. Click to be taught extra in regards to the product.

Why sequence your monoclonal antibody (mAb)?
  • Patent Application – If you intend to acquire a patent to your mAb, antibody sequencing will likely be obligatory to be able to distinguish your novel antibody from others.
  • Antibody Recombination – To decide the sequence of a mAb, the cDNA of your antibody may be remoted from a hybridoma cell line and its subcloning and expression in mammalian cells.
  • Antibody Humanization – Antibody sequence information is important for any antibody engineering or humanization improvement.
FITC ANTI-RABIES MONOCLONAL GLOBULIN

FITC ANTI-RABIES MONOCLONAL GLOBULIN

Oak BioSciences presents quick and dependable cloning and sequencing providers for monoclonal antibodies. Our distinctive expertise permits us to sequence your antibodies from any antibody-producing cell line (hybridomas, peripheral blood lymphocytes, B-cells, spleen cells or bone marrow), and from a wide range of species (mouse, rat, hamster, rabbit, hen, camelids, cartilaginous fishes, human and different primates). The service may be tailor-made to particular parameters, from variable areas or domains to full-length antibody sequencing.

The FDI FITC ANTI-RABIES MONOCLONAL GLOBULIN is used within the direct fluorescent antibody process for the in vitro detection of rabies in brains and submaxillary glands.The direct fluorescent antibody take a look at is the most effective single take a look at obtainable for the analysis of rabies. Studies have proven that the fluorescent antibody methodology for detection of rabies could also be extra delicate than the mouse inoculation take a look at;nonetheless, the outcomes of the 2 exams are 99-100% correlative.

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