BackgroundHemocyanins are proteins that use copper binding websites to bind and transport oxygen in a wide range of arthropods and mollusks. Hemocyanin is remoted from the hemolymph of the animals. Hemocyanin is likely one of the strongest antigens recognized. Hemocyanin has been in use as an immunological reagent for a few years.
It is used as a provider protein for antibody manufacturing in opposition to antigens. Recent advances in immunology and the position immune system performs in ailments have opened an entire new period of product improvement actions aimed toward creating novel therapeutics which is aimed toward educating the physique’s immune system to battle ailments like most cancers, AIDS, and so forth.
The strategy entails using extremely immunogenic molecule just like the hemocyanin for non-specific immunostimulation (NSI) or energetic particular immunostimulation (ASI) utilizing conjugate vaccines, whereby the tumor (illness) particular antigens are covalently sure to provider protein like KLH and the product utilized in human scientific research. Such merchandise are termed “vaccines”. BioVision’s KLH subunits powder has main benefits related to it, when it comes to flexibility of use, the selection of buffer in early developmental research and avoidance of points related to reconstitution of dry powder. These subunits are extremely pure and have low endotoxin content material.
megathura-crenulata
| Gene Symbol | KLH |
|---|---|
| Other Names | KLH, keyhole limpett hemocyanin, hemocyanin, Megathura crenulata hemocyanin |
| Gene Source | Megathura crenulata |
| Source | Megathura crenulata, Giant keyhole limpet |
| Assay&Purity | MPLC-SEC; ≥ 95% |
| Assay2&Purity2 | N/A; |
| Recombinant | No |
| Application Notes | Resuspend in sterile water to a 1 mg/ml focus |
| Format | Lyophilized protein |
| Storage | 2-8°C ; Freeze Dried |
General description
Gastropod hemocyanins are large glycoproteins (four to eight MDa) designed by an intricate association of 10 subunits which might be self-assembled into hole cylinders 35 nm in diameter.
Hemocyanin is a excessive molecular weight copper containing glycoprotein. It reversibly binds oxygen and varieties the extra-cellular respiratory protein of mollusks. Keyhole limpet hemocyanin (KLH) is filtered from the hemolymph of Megathura crenulata, additionally referred to as the large keyhole limpet. It is native to the southern California coast and Mexico.
Application
Keyhole limpet hemocyanin (KLH) is a robust and well-known immune-stimulant in each experimental animals and people. It has been used as a hapten provider protein to boost antigen-specific T cell priming and is understood to stimulate a CD4+ T-cell response.
Hemocyanin was used to review if antipeptide antibodies particularly acknowledge the l1 protein of the human papilloma virus of kind 31. It was additionally used to review the acid-induced unfolding of didecameric keyhole limpet hemocyanin.
Hemocyanin was used to review if antipeptide antibodies particularly acknowledge the l1 protein of the human papilloma virus of kind 31. It was additionally used to review the acid-induced unfolding of didecameric keyhole limpet hemocyanin.
Physical kind
Solution in PBS, pH 7.2, containing 1mM CaCl2 and 0.5 mM MgCl2 with 15 mM sodium azide as preservative
Preparation Note
Chromatographically purified.
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