Hemocyanins are proteins that use copper binding websites to bind and transport oxygen in a wide range of arthropods and mollusks. Hemocyanin is remoted from the hemolymph of the animals. Hemocyanin is likely one of the strongest antigens recognized.
Hemocyanin might be use as an immunological reagent for a few years. It is used as a provider protein for antibody manufacturing in opposition to antigens. Recent advances in immunology and the position immune system performs in ailments have opened an entire new period of product improvement actions aimed toward creating novel therapeutics for educating the physique’s immune system to battle ailments. The strategy entails using extremely immunogenic molecule just like the hemocyanin for non-specific immunostimulation (NSI) or energetic particular immunostimulation (ASI) utilizing conjugate vaccines, whereby the tumor (illness) particular antigens are covalently sure to provider protein like KLH and the product utilized in human scientific research.
Native KLH has low endotoxin content material and constant excessive focus in a phosphate buffer containing no extraneous steel ions for product stabilization, nevertheless with improved stability. This analysis grade materials is suited to use in vaccine product improvement actions and in addition for routine immunological research, antibody manufacturing, manufacturing of activated KLH and different developmental actions.
BackgroundHemocyanins are proteins that use copper binding websites to bind and transport oxygen in a wide range of arthropods and mollusks. Hemocyanin is remoted from the hemolymph of the animals. Hemocyanin is likely one of the strongest antigens recognized. Hemocyanin has been in use as an immunological reagent for a few years.
It is used as a provider protein for antibody manufacturing in opposition to antigens. Recent advances in immunology and the position immune system performs in ailments have opened an entire new period of product improvement actions aimed toward creating novel therapeutics which is aimed toward educating the physique’s immune system to battle ailments like most cancers, AIDS, and so forth.
The strategy entails using extremely immunogenic molecule just like the hemocyanin for non-specific immunostimulation (NSI) or energetic particular immunostimulation (ASI) utilizing conjugate vaccines, whereby the tumor (illness) particular antigens are covalently sure to provider protein like KLH and the product utilized in human scientific research. Such merchandise are termed “vaccines”. BioVision’s KLH subunits powder has main benefits related to it, when it comes to flexibility of use, the selection of buffer in early developmental research and avoidance of points related to reconstitution of dry powder. These subunits are extremely pure and have low endotoxin content material.
megathura-crenulata

megathura-crenulata

Additional Info
Gene Symbol KLH
Other Names KLH, keyhole limpett hemocyanin, hemocyanin, Megathura crenulata hemocyanin
Gene Source Megathura crenulata
Source Megathura crenulata, Giant keyhole limpet
Assay&Purity MPLC-SEC; ≥ 95%
Assay2&Purity2 N/A;
Recombinant No
Application Notes Resuspend in sterile water to a 1 mg/ml focus
Format Lyophilized protein
Storage 2-8°C ; Freeze Dried

General description

Gastropod hemocyanins are large glycoproteins (four to eight MDa) designed by an intricate association of 10 subunits which might be self-assembled into hole cylinders 35 nm in diameter.
Hemocyanin is a excessive molecular weight copper containing glycoprotein. It reversibly binds oxygen and varieties the extra-cellular respiratory protein of mollusks. Keyhole limpet hemocyanin (KLH) is filtered from the hemolymph of Megathura crenulata, additionally referred to as the large keyhole limpet. It is native to the southern California coast and Mexico.

Application

Keyhole limpet hemocyanin (KLH) is a robust and well-known immune-stimulant in each experimental animals and people. It has been used as a hapten provider protein to boost antigen-specific T cell priming and is understood to stimulate a CD4+ T-cell response.
Hemocyanin was used to review if antipeptide antibodies particularly acknowledge the l1 protein of the human papilloma virus of kind 31. It was additionally used to review the acid-induced unfolding of didecameric keyhole limpet hemocyanin.

Physical kind

Solution in PBS, pH 7.2, containing 1mM CaCl2 and 0.5 mM MgCl2 with 15 mM sodium azide as preservative

Preparation Note

Chromatographically purified.

Keyhole Limpet Hemocyanin (Megathura Crenulata) Carrier protein

KLH51-R10 10 mg
EUR 202

Keyhole Limpet Hemocyanin (Megathura Crenulata) Carrier protein

KLH51-R100 100 mg
EUR 408

Keyhole Limpet Hemocyanin (KLH, Megathura Crenulata, >98%, low endotoxin, azide free)

KLH52-R-LE-1 1 mg
EUR 225

PMA

40013 1MG
EUR 196
Description: Minimum order quantity: 1 unit of 1MG

PMA

20-abx076524
  • EUR 244.00
  • EUR 439.00
  • 1 mg
  • 5 mg
  • Shipped within 5-12 working days.

pMA-SpCas9-g3

PVT17837 2 ug
EUR 300

pMA-SpCas9-g4

PVT17838 2 ug
EUR 300

pMA-SpCas9-g5

PVT17843 2 ug
EUR 300

pMA-SpCas9-g2

PVT17844 2 ug
EUR 300

pMA-SpCas9-g1

PVT17848 2 ug
EUR 300

pMA-SpCas9-g9

PVT17849 2 ug
EUR 300

pMA-SpCas9-g8

PVT17850 2 ug
EUR 300

pMA-SpCas9-g10

PVT17852 2 ug
EUR 300

pMA-SpCas9-g7

PVT17854 2 ug
EUR 300

pMA-SpCas9-g6

PVT17855 2 ug
EUR 300

PMA (PKC kinase activator)

SIH-508-1MG 1 mg
EUR 126
  • PMA (Phorbol 12-myristate 13-acetate) is a phorbol ester. It is used experimentally as a reversible protein kinase C (PKC) activator. It has been shown to be a potent tumor promoter.
Description: The substance PMA is a pkc kinase activator. It is synthetically produced and has a purity of ?99%. The pure substance is colorless amorphous film or foam which is soluble in DMSO (25 mg/ml), 100% ethanol (25 mg/ml), acetone, ether or dimethyl formamide; almost insoluble in aqueous buffers.

PMA (PKC kinase activator)

SIH-508-5MG 5 mg
EUR 266
  • PMA (Phorbol 12-myristate 13-acetate) is a phorbol ester. It is used experimentally as a reversible protein kinase C (PKC) activator. It has been shown to be a potent tumor promoter.
Description: The substance PMA is a pkc kinase activator. It is synthetically produced and has a purity of ?99%. The pure substance is colorless amorphous film or foam which is soluble in DMSO (25 mg/ml), 100% ethanol (25 mg/ml), acetone, ether or dimethyl formamide; almost insoluble in aqueous buffers.

PMA-Lite LED Photolysis Device

E90002 1EA
EUR 2211
Description: Minimum order quantity: 1 unit of 1EA

Viability PCR Starter Kit with PMA

31075 1kit
EUR 363
Description: Minimum order quantity: 1 unit of 1kit