Key choices and particulars


  • Sensitivity: 10 pg/ml
  • Vary: 78 pg/ml – 5000 pg/ml
  • Pattern kind: Cell custom supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay kind: Sandwich (quantitative)
  • Reacts with: Human


  • Product determine

    Human BAFF-R ELISA Equipment
  • Detection method

  • Precision

    Pattern n Imply SD CV%
    1 16 147pg/ml 10.29 = 7%
    2 16 683pg/ml 49.85 = 7.3%
    3 16 2750pg/ml 206.25 = 7.5%
    Pattern n Imply SD CV%
    1 24 156pg/ml 13.88 = 8.9%
    2 24 700pg/ml 62.3 = 8.9%
    3 24 2531pg/ml 227.05 = 8.9%
    bio human baff r

    bio human baff r

  • Pattern kind

    Cell custom supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
  • Assay kind

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Vary

    78 pg/ml – 5000 pg/ml
  • Assay time

    3h 30m
  • Assay size

    Various steps commonplace assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) gear (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell custom supernatants, cell lysates, serum and plasma (heparin, EDTA).

    The ELISA gear is based on commonplace sandwich enzyme-linked immune-sorbent assay experience. A monoclonal antibody from mouse explicit for BAFF-R has been pre-coated onto 96-well plates. Requirements (Expression system for regular: NSO; Immunogen sequence: S7-A71) and check out samples are added to the wells, a biotinylated detection polyclonal antibody from goat explicit for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complicated is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualise HRP enzymatic response. TMB is catalyzed by HRP to produce a blue shade product that grew to become yellow after together with acidic stop reply. The density of yellow is proportional to the Human BAFF-R amount of sample captured in plate.

  • Notes

    BAFF-R (additionally known as Tumor necrosis subject receptor superfamily member 13C or B-cell-activating subject receptor)  is a protein that in individuals is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating subject (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a sort III transmembrane protein containing a single extracellular phenylalanine-rich space. It’s thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

  • Platform

    Pre-coated microplate (12 x Eight correctly strips)

[Linking template=”default” type=”products” search=”human baff r” header=”2″ limit=”26″ start=”1″ showCatalogNumber=”true” showSize=”true” showSupplier=”true” showPrice=”true” showDescription=”true” showAdditionalInformation=”true” showImage=”true” showSchemaMarkup=”true” imageWidth=”” imageHeight=””]